OR2W3在胃腺癌中的表达及其意义

Expression and significance of OR2W3 in gastric adenocarcinoma

目的检测分析胃腺癌中嗅觉受体家族2亚家族W成员3(olfactory receptor family 2 subfamily W member 3,OR2W3)的表达及意义,探讨其与细胞增殖、凋亡的相关性。方法选择2013年1月至2014年6月在华北理工大学附属医院确诊为胃腺癌并行根治手术的患者61例作为研究对象,选择肿瘤组织61例作为观察组,选择正常胃黏膜组织61例作为对照组。免疫组化法检测两组中OR2W3、观察组中HER-2蛋白的表达,对部分HER-2表达不确定者应用荧光原位杂交法(FISH)检测HER-2基因扩增情况,Western Blot法检测观察组中增殖细胞核抗原(PCNA)和B细胞淋巴瘤-2相关X蛋白(BAX)的表达。结果OR2W3观察组中的阳性率50.8%(31/61)高于对照组8.2%(5/61),两组比较差异有统计学意义(χ^2=26.63,P<0.05)。观察组中OR2W3的阳性率在不同肿物最大径[≥6 cm为68.8%(22/32),<6 cm为31.0%(9/29)]、浸润深度(浆膜及以外为63.4%(26/41),未及浆膜为25.0%(5/20))、血管淋巴管累犯(有累犯为78.6%(11/14),无累犯为42.6%(20/47))、淋巴结转移(有转移为82.1%(23/28),无转移为24.2%(8/33))和TNM分期(Ⅲ+Ⅳ期为78.1%(25/32),Ⅰ+Ⅱ期为20.7%(6/29))的差别有统计学意义(χ^2值分别为8.658、7.937、5.559、20.318、20.080,P均<0.05)。OR2W3的表达与生存时间有关(χ^2=5.31,P<0.05)。OR2W3与PCNA的表达具有正相关性(r=0.54,P<0.05),OR2W3与BAX的表达具有负相关性(r=-0.59,P<0.05)。HER-2阳性共8例,阴性共53例,OR2W3在不同HER-2表达的肿瘤中差别有统计学意义(χ^2=4.957,P<0.05)。结论OR2W3在胃腺癌组织中表达明显升高,可以促进肿瘤细胞的增殖、抑制细胞凋亡,OR2W3与HER-2表达相关,检测OR2W3的表达对判断预后可能有一定价值。

Objective To detect and analyze the expression and significance of olfactory receptor family 2 subfamily W member 3(OR2W3)in gastric adenocarcinoma,and to explore its correlation with cell proliferation and apoptosis.Methods From January 2013 to June 2014,61 patients with gastric adenocarcinoma diagnosed in the Affiliated Hospital of North China University of Technology and undergoing radical operation were selected as the study subjects.Neoplasm tissue were selected as the observation group,normal gastric mucosa(61 cases)were selected as the control group.Immunohistochemistry was used to detect the expression of or2w3 and HER-2 in the two groups.Fish was used to detect the amplification of HER-2 gene in some of the patients with uncertain expression of HER-2.Western blot was used to detect the expression of PCNA and BAX in the observation group.Results Positive rate of OR2W3 was higher in the observation group(50.8%,31/61)than that in the control group(8.2%(5/61)),and the difference between the two groups was statistically significant(χ^2=26.63,P<0.05).The difference of the positive rate of OR2W3 in the maximum diameter(≥6 cm was 68.8%(22/32),<6 cm was 31.0%(9/29)),depth of invasion(seroas and beyond was 63.4%(26/41),less serosa was 25.0%(5/20)),vascular and lymphatic vessel involvement(involvement was 78.6%(11/14),no involvement was 42.6%(20/47)),lymph node metastasis(metastasis was 82.1%(23/28),no metastasis was 24.2%(3/33))and TNM staging(Ⅲ+Ⅳwas 78.1%(25/32),Ⅰ+Ⅱwas 20.7%(6/29))in observation group was statistically significant(χ^2 was 8.423,7.937,5.559,20.318,20.080,respectively,all P<0.05).Expression of OR2W3 was correlated with survival time(X2=5.31,P<0.05).Positive correlation was found between OR2W3 and PCNA expression(r=0.54,P<0.05).Negative correlation were found between OR2W3 and BAX expression(r=-0.59,P<0.05).There were 8 cases of HER-2 positive and 53 cases of HER-2 negative.The difference of OR2W3 expression in tumors with different HER-2 expression was statistically significant(χ^2=4.957,P<0.05).Conclusion The expression of OR2W3 in gastric adenocarcinoma tissue is significantly increased,which can promote the proliferation of cancer cells and inhibit apoptosis.OR2W3 is related to HER-2 expression.Detection of OR2W3 expression may be of some value in judging prognosis.