高粱抗丝黑穗病菌4号生理小种SRAP标记分析

SRAP Marker Analysis of Sorghum Germplasm Resistance to Race 4 of Head Smut

高粱丝黑穗病是全世界普遍存在的重要高粱病害,该病会对高粱穗部的结构造成破坏,导致高粱减产,甚至颗粒无收。为培育高粱抗丝黑穗病新品种,筛选与抗病基因相关的分子标记,实现真正意义上的分子标记辅助选择育种,以高粱感病品种三尺三与抗病品系961541为研究材料,以F2群体为定位群体,采用BSA方法筛选与抗丝黑穗病基因相关的SRAP标记。结果表明,感病亲本三尺三的发病率为37.5%,抗病亲本961541的发病率为0,F1的发病率为0;田间种植的F2群体共211株,其中,感病植株16株,抗病植株195株,发病率为7.58%,F2群体抗感植株比率接近15∶1(χ^2值分别为0.027,0.406),推测4号生理小种受2对非等位基因控制;289对SRAP引物中有119对引物在亲本间表现差异,119对引物中有9对引物在抗感池间表现差异,抗池的条带与抗病材料961541带型一致,感池的条带与感病材料三尺三带型一样。经抗感单株验证,结果显示,仅有1对SRAP引物(Em4/Me6)存在差异。

Head smut is one of the most important sorghum diseases around the world.This disease can damage the structure of sorghum panicle,resulting in seriously reduced grain production or even no harvest.For breeding new varieties resistant to head smut,screening molecular markers associated to resistance gene in sorghum,and achieving the real molecular marker-assisted breeding,a sensitive sorghum variety Sanchisan and a resistant variety 961541 were selected as research materials,the SRAP markers related to head smut resistance were screened by bulked segregation analysis(BSA)method using the F2 population.The results showed that the incidence of sensitive parent Sanchisan was 37.5%,the incidence of resistance parent 961541 was 0,the incidence of F1 generation was 0.The F2 population had a total of 211 plants,of which 16 plants were sensitive and 195 plants were resistant,with an incidence of 7.58%.The incidence of F2 closed to 15∶1(χ^2=0.027,0.406),it was speculated that resistance genes to head smut race 4 should be controlled by 2 non-allelic genes.Among 289 pairs SRAP primers,119 primers showed differences in parents,9 pairs primers showed differences between resistant pool and sensitive pool.The banding pattern of resistance pool was the same as 961541,and the sensitive pool was as the material Sanchisan.The results of verified showed that only one pair SRAP primer(Em4/Me6)showed differences in resistant plant and sensitive plant.