光质对刺芹侧耳原基发生期分化及生理效应的影响

Effects of light conditions on the differentiation and physiological effects of Pleurotus eryngii primordium

为探索光质对刺芹侧耳原基发生的影响,将长满菌丝体的菌包进行不同光质(红光、黄光、绿光、蓝光、白光、黑暗)处理,观察记录原基分化及子实体生长状况,并测定原基分化过程中的胞外酶活性和海藻糖含量.结果显示:不同光质条件下原基发生时间略有差异,菇蕾分化差异明显;与工厂化生产常用的白光照射条件相比,绿光处理组中子实体的农艺性状更优,菌柄直径和长度均加长,菌盖直径相应变大,但生物学效率差异不显著;不同光质处理对羧甲基纤维素酶、半纤维素酶活性的影响不够显著,对淀粉酶的影响较大.原基分化期红光、绿光、白光、黑暗4个处理组中的海藻糖含量均呈现下降趋势;黄光与蓝光处理组中该指标的变化相似,在原基形成时出现一个极大值,具体缘由有待进一步探究.本研究表明绿光可以作为刺芹侧耳工厂化生产的首选光质.

To evaluate the effects of different light conditions on primordium differentiation of Pleurotus eryngii,cultivation bags fully covered with mycelia were treated with different light conditions(red light,yellow light,green light,blue light,white light,and no light).Primordium differentiation and growth status of the fruiting bodies were evaluated,and extracellular enzyme activity and trehalose content were determined during primordium differentiation.The results showed that primordium formation time was slightly different under different light conditions,but differentiation status was markedly different However,compared with white light conditions commonly used in factory production,the agronomic traits of the fruiting bodies were improved in the green light treatment group.Under green light,the stalks were longer and had a larger diameter;moreover,the caps had a larger diameter.However,there was no significant difference in biological efficiency.The different light treatments did not significantly affect carboy me thy lcellulase or hemicellulase activity,but amylase activity was significantly affected.Trehalose content showed a downward trend during the primordial differentiation periodin the red,green,and white light and dark treatment groups.A similar change in trehalose content was seen with blue light treatment,reaching a maximum value at primordium formation.Understanding the specific reason for this requires further investigation.The results of this study showed that green light can be used as the preferred light condition for the factory production of P.eryngii.