四川大叶黄精组培快繁技术

Investigating tissue culture technique of a Polygonatum kingianum variety distributed in Sichuan

黄精市场需求量不断增加,较低的自然繁殖速度难以满足人们的需求.通过组织培养技术,建立黄精快速繁殖技术体系,可以高效获得大量优质种苗在生产上应用.首次以四川主载品种大叶黄精地下根茎芽为外植体进行组培快繁体系的初步研究.结果显示:外植体消毒先用0.5 g/L的多菌灵溶液预处理12 h,再用0.2%HgCl2灭菌6 min+6 min,污染率可得到有效控制;四川大叶黄精根茎芽最适宜的初代培养基为MS+6-BA 0.5 mg/L+NAA 0.5 mg/L,萌发率达35%,长势健壮;不定芽分化培养基为MS+6-BA 3.0 mg/L+NAA 0.2 mg/L,平均不定芽诱导个数为4.33个;壮苗培养基为MS+6-BA 1.5 mg/L+2,4-D 0.5 mg/L+GA30.5 mg/L;生根培养基为1/2 MS+NAA 1.0 mg/L+IBA 0.2 mg/L+AC 0.2 g/L,生根率达89%.本研究表明以大叶黄精的地下根茎芽为外植体可快速获得大量组培苗,其中细胞分裂素6-BA在大叶黄精生长过程中起着重要作用,结果可为四川提供便捷优质大叶黄精供苗途径.

Current methods to propagate Polygonatum sibiricum are struggling to meet the rapidly increasing demand for Polygonatum.In order to provide high quality P.kingianum Coll.et Hemsl.var.grandifolium D.M.Liu et W.Z.Zeng,predominant cultivars of P.sibiricum in Sichuan,we developed a rapid micropropagation system using rhizome buds as explants.The results show that the contamination rate can be effectively controlled when the explants were pretreated with0.5 g/L carbendazim solution for 12 h,before being sterilized with 0.2% HgCl2 for 6 min+6 min.The optimal medium for primary culture was MS(Murashige and Skoog)+6-BA 0.5 mg/L+NAA 0.5 mg/L;the induction rate for this medium was35.0%.The optimal medium for adventitious bud induction was MS+6-BA 3.0 mg/L+NAA 0.2 mg/L;the optimal medium for cultivating strong seeding was MS+6-BA 1.5 mg/L+2,4-D 0.5 mg/L+GA3 0.5 mg/L;the medium 1/2 MS+NAA 1 mg/L+IB A 0.2 mg/L+AC 0.2 g/L was suitable for the rooting and the rooting rate was 89%.A large number of tissue culture plantlets can be obtained quickly using the rhizome buds of P.sibiricum as explants.The cytokinin 6-BA plays an important role in the growth of P.sibiricum.