亚临界流体萃取制备苦水玫瑰净油工艺及其关键香气化合物的研究

Study on Subcritical Fluid Extraction Technology for Absolute of Ku-Shui Rose and the Key Aroma Components in This Oil

以苦水玫瑰为原料,通过三因素三水平正交试验优化亚临界丁烷萃取制备玫瑰净油工艺,以玫瑰浸膏萃取率为指标,得到最佳萃取工艺条件为:萃取温度50℃,萃取次数8次,萃取时间75 min。在该条件下玫瑰浸膏萃取率可达2.56%。所得玫瑰净油利用计算机标准谱库匹配检索鉴定了47种化合物,占总峰面积的90.48%,其中主要香气特征成分有苯甲醇(33.67%)、丁香酚(11.04%)、2-十三烷酮(5.66%)、β-苯乙醇(5.60%)、香茅醇(4.11%)、香叶醇(3.68%)等,它们是导致不同玫瑰净油香气差异的关键香气化合物。

Using Ku-shui rose as raw material, the subcritical butane extraction technology for rose absolute was optimized by three-factor and three-level orthogonal test, and the yield of rose extract as the index, the best extraction process conditions were:extraction temperature 50 ℃,frequency of extraction 3 times, and extraction time 75 min. Under this condition, the highest extract yield can reach to 2.47%. Based on computer standard spectrum data, 47 volatile components were identified, their peak areas were accounted to 90.48% of the total peak area. The main aroma components included benzyl alcohol(33.76%), eugenol(11.04%), 2-tridecanone(5.66%), β-phenethyl alcohol(5.60%), citronellol(4.11%), geraniol(3.68%), etc., they are the key aroma components resulting different aroma characteristics of different rose absolutes.