NF-κBp65蛋白和自噬相关因子Beclin1及p62在甲状腺癌中的表达及临床意义

The expressions and clinical significance of NF-κBp65 and autophagy related proteins Beclin1 and p62 in thyroid papillary carcinoma

目的分析核因子NF-κB(NF-κB)p65和自噬相关蛋白Beclin1及p62在甲腺乳头状癌(PTC)中的表达及其临床意义。方法收集2013年3月至2015年2月期间笔者所在医院收治的160例经病理学检查证实为PTC患者的肿瘤组织标本及其癌旁组织标本,同时收集上述患者中伴有颈部淋巴结转移组织标本90例。采用免疫组化方法检测PTC组织、癌旁组织和转移淋巴结组织中NF-κBp65、Beclin1及p62的表达情况,分析上述指标与PTC患者临床病理特征及预后的关系。结果 NF-κBp65和p62在PTC组织和转移淋巴结组织中的表达阳性率均高于癌旁组织(P<0.05),而Beclin1在PTC组织和转移淋巴结组织中的表达阳性率均低于癌旁组织(P<0.05)。PTC组织中NF-κBp65的表达与患者的临床病理特征均无关(P>0.05);p62的表达随肿瘤分化程度升高而降低(P<0.05);Ⅲ+Ⅳ期和有淋巴结转移患者中的Beclin1表达分别低于Ⅰ+Ⅱ期和无淋巴转移者(P<0.05),而p62的表达与之相反。Spearman相关分析显示,PTC组织中,Beclin1和p62的表达呈负相关关系(r=–0.656,P<0.01),在转移淋巴结组织中,Beclin1和p62的表达也呈负相关关系(r=–0.562,P<0.01)。PTC组织中p62及NF-κBp65表达阳性者3年生存率均低于表达阴性者(P<0.05),Beclin1表达阳性者3年生存率则高于表达阴性者(P<0.05)。TNM分期、淋巴结转移、NF-κBp65及p62为PTC预后的独立危险因素,而Beclin1为保护因素。结论NF-κBp65和p62在PTC组织及淋巴结转移组织中呈高表达,而Beclin1呈低表达,三者可作为PTC患者预后独立预测因素;Beclin1及p62与PTC生物学行为有关,可能成为PTC诊断的潜在指标。

Objective To analyze the expression and significance of NF-κBp65 and autophagy-related proteins Beclin1 and p62 in patients with papillary thyroid carcinoma(PTC).Methods One hundred and sixty cases of PTC patients'tumor tissue specimens and paracancerous tissue specimens in our hospital from March 2013 to February 2015 were collected,and 90 cases of cervical lymph node metastasis tissue specimens of the above patients were collected.The expressions of NF-κBp65,Beclin1 and p62 in PTC tissues,metastatic lymph node tissues and paracancerous tissues were detected by immunohistochemical method,and the relationship between the above indexes and the clinicopathological characteristics and prognosis of PTC patients was analyzed.Results The positive rates of expression of NF-kappa Bp65 and p62 in PTC tissues and metastatic lymph node tissues were higher than those in paracancerous tissues(P<0.05).The expression rate of Beclin1 in PTC tissues and metastatic lymph node tissues was lower than that in paracancerous tissues(P<0.05).The positive rate of NF-κBp65 expression in PTC tissues was not related to the clinicopathological characteristics of patients(P>0.05).The expression of p62 decreased with the increase of tumor differentiation(P<0.05).The expression of Beclin1 in patients with stageⅢ+Ⅳand lymph node metastasis were lower than those in patients with stageⅠ+Ⅱand without lymph node metastasis(P<0.05),while the expression of p62 was opposite.Spearman correlation analysis showed that the expression of Beclin1 and p62 in PTC tissues was negatively correlated(r=–0.656,P<0.01).In metastatic lymph node tissues,the expression of Beclin1 and p62 was also negatively correlated(r=–0.562,P<0.01).The 3-year survival rates of patients with positive expression of p62 and NF-κBp65 in PTC tissues were lower than that of patients with negative expression(P<0.05).The 3-year survival rate of patients with positive expression of Becrin1 was higher than that of negative expression(P<0.05).TNM stage,lymph node metastasis,NF-κBp65 and p62 were independent risk factors for PTC prognosis,and Beclin1 was protective factor.Conclusions NF-κBp65 and p62 are highly expressed in PTC tissues and lymph node metastasis tissues,while Beclin1 is poorly expressed,which could be used as independent prognostic factors for PTC patients.In addition,Beclin1 and p62 are related to PTC biological behavior and may become potential indicators for PTC diagnosis.