高效液相色谱法同时测定麻仁丸中大黄素、大黄酚、大黄酸及厚朴酚含量

Simultaneous determination of emodin, chrysophanol, parietic acid, and magnolol in Fructus Cannabis Bolus by high performance liquid chromatography

目的 建立同时测定麻仁丸中大黄素、大黄酚、大黄酸、厚朴酚含量的 HPLC 方法.方法 采用奥泰ALLTIMA-C18色谱柱(250 mm×4.6 mm,5 μm);流动相为甲醇(A)-0.1%磷酸水溶液(B),梯度脱洗;流速1.0 ml/min;柱温30 ℃;进样量10 μl;检测波长254 nm.结果 大黄素、大黄酚、大黄酸、厚朴酚4种有效成分线性范围分别为9.05-81.26 μg(r=0.999 8)、13.28-119.30 μg(r=0.997 6)、8.30-75.40 μg (r=0.998 7)、6.30-61.79 μg(r=0.996 5);大黄素、大黄酚、大黄酸、厚朴酚平均回收率分别为99.25%、99.46%、99.51%、99.86%,RSD分别为2.04%、3.23%、1.84%、2.42%.结论 所建立的方法操作简便快捷,重复性好,可同时测定麻仁丸中大黄素、大黄酚、大黄酸、厚朴酚4种有效成分含量.

Objective To simultaneously determinate 4 effective components in Fructus Cannabis Bolus by high performance liquid chromatography (HPLC). Methods The column was ALLTIMA-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol (A)-0.1% phosphoric acid aqueous solution (B) in gradient elution program. The flow rate was 1.0 ml/min, the column temperature was 30℃, and the injection volume was 10 μl. The detection wavelength was set at 254 nm. Results The linear ranges of 4 effective components of emodin, chrysophanol, parietic acid, and magnolol were 9.05-81.26 μg (r=0.999 8), 13.28-119.3 μg (r=0.997 6), 8.30-75.40 μg (r=0.998 7), 6.30-61.79 μg (r=0.996 5), respectively;the average recoveries and RSDs of emodin, chrysophanol, parietic acid, and magnolol were 99.25%, 99.46%, 99.51%, 99.86%, respectively. The RSDs of emodin, chrysophanol, parietic acid, and magnolol were 2.04%, 3.23%, 1.84%, and 2.42%, respectively. Conclusions The HPLC can be used as an effective and feasible method for the determination of emodin, chrysophanol, parietic acid, and magnolol in Fructus Cannabis Bolus. The method is simple, quick, and reproducible, and can be used for the quality control of Fructus Cannabis Bolus.