黄芪多糖预处理对肾缺血再灌注损伤保护作用

Protective effect of astragalus polysac charides on renal ischemial reperfusion injury

目的肾缺血再灌注损伤(renal ischemical reperfusion injury,RIRI)是临床常见危重病理现象,是诱发肾脏功能损伤乃至肾衰竭的重要危险因素。本研究探讨黄芪多糖(astragalus polysac charides,APS)对RIRI影响。方法SD大鼠30只随机分为假手术组、模型组和APS干预组,每组10只。APS干预组在建模前,每天给予APS 450mg/kg灌胃,连续用药10d,1次/d;假手术组和模型组以相同方式灌胃等体积的生理盐水。模型组与APS干预组建模,假手术组只分离不建模,48h后肾动脉采血,处死大鼠并取肾脏组织。HE染色观察肾组织结构变化,全自动生化分析仪测定血清中血尿素氮(blood urea nitrogen,BUN)和肌酐(creatinine,Cr)水平,分光光度计检测肾组织超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdeyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)水平。结果假手术组肾脏形态结构正常,模型组观察到损伤及坏死,APS干预组肾脏损伤及坏死减少。假手术组、模型组和APS干预组BUN水平分别为(9.25±1.32)、(16.59±2.31)和(12.39±1.88)mmol/L,差异有统计学意义,F=38.34,P<0.05;Cr水平分别为(152.36±26.28)、(512.65±69.32)和(213.68±31.29)μmol/L,差异有统计学意义,F=172.16,P<0.05。假手术组、模型组和APS干预组SOD水平分别为(2.35±0.38)、(0.48±0.12)和(1.99±0.33)U/mL,差异有统计学意义,F=110.32,P<0.05;假手术组、模型组和APS干预组MDA水平分别为(1.84±0.23)、(6.69±0.82)和(2.38±0.35)nmol/mg,差异有统计学意义,F=250.00,P<0.05;假手术组、模型组和APS干预组GSH-PX水平分别为(130.28±14.39)、(48.29±9.65)和(102.59±9.98)U/mL,差异有统计学意义,F=130.54,P<0.05。结论APS预处理对肾缺血再灌注损伤具有保护作用,肾脏结构明显改善,且血清BUN和Cr水平降低,明显提高肾脏功能,其机制可能与抗氧化应激有关。

OBJECTIVE Renal ischemia reperfusion injury(RIRI)is a common and critical pathological phenomenon in clinic.And RIRI is an important risk factor for renal function damage.This study aims to investigate the effect and mechanism of astragalus polysac charides(APS)on RIRI.METHODS Thirty SD rats were randomly divided into sham operation group,model group and APS intervention group.APS intervention group were intragastric administration with APS 450 mg/kg once a day for 10 days,before the model was established.The Sham operated group and model group were intragastric administration with the same volume of saline.The model group and APS intervention group were established the model,but the sham operation group were not.After 48 hours,renal artery blood was collected and kidney tissues were taken when the rats were killed.Renal tissue structure were observed by HE staining.Blood urea nitrogen(BUN),creatinine(Cr)in the serum were measured by automatic biochemical analyzer.Superoxide dismutase(SOD),malondialdeyde(MDA),glutathione peroxidase(GSH-PX)in renal tissue were detected by spectrophotometer.RESULTS The shape and structure of kidney in sham operation group were normal.Injury and necrosis were observed in model group.Renal injury and necrosis were reduced in APS intervention group.The level of BUN in the sham operation group,the model group and the APS intervention group were(9.25±1.32),(16.59±2.31)and(12.39±1.88)mmol/L,effectively,F=38.34,P<0.05;The level of Cr were(152.36±26.28),(512.65±69.32)and(213.68±31.29)μmol/L,effectively,F=172.16,P<0.05.The level of SOD in the sham operation group,the model group and the APS intervention group were(2.35±0.38),(0.48±0.12)and(1.99±0.33)U/mL,effectively,F=110.32,P<0.05;The level of MDA were(1.84±0.23),(6.69±0.82)and(2.38±0.35)nmol/mg,effectively,F=250.00,P<0.05;The level of GSH-PX were(130.28±14.39),(48.29±9.65)and(102.59±9.98)U/mL,effectively,F=130.54,P<0.05.CONCLUSIONS APS pretreatment has protective effect on RIRI.The structure of kidney is improved obviously,and the contents of BUN and CR in serum are decreased significantly,which can improve renal function.The mechanism may be related to antioxidant stress.