Sorcin表达水平影响卵巢癌患者化学药物治疗耐药及生存时间

Level of Sorcin expression influences chemoresistance and overall survival in patients with ovarian cancer

目的:探讨可溶性耐药相关钙结合蛋白质(soluble resistance-related calcium-binding protein,Sorcin)与卵巢癌化学药物治疗(以下简称化疗)耐药的关系及对患者生存时间的影响。方法:采用免疫组织化学方法检测27例卵巢癌化疗耐药和37例卵巢癌化疗敏感患者组织中Sorcin的表达情况,分析Sorcin与卵巢癌患者化疗耐药及临床病理特征的相关性;采用Log-rank检验对患者生存时间与Sorcin进行单因素影响分析;采用Cox回归模型分析各临床病理因素与生存时间之间的关系;采用Kaplan-Meier方法分析化疗耐药组与敏感组的生存率。结果:卵巢癌化疗耐药组的Sorcin免疫组织化学半定量评分显著高于敏感组(P<0.001)。64例卵巢癌患者Sorcin的表达在不同年龄组、有无腹水组、有无大网膜转移组、不同临床分期、不同病理分级和组织类型组间的比较差异均无统计学意义(P>0.05)。64例卵巢癌患者的Sorcin表达与生存时间呈负相关(r=–0.326,P<0.05)。Log-rank检验显示Sorcin的表达升高对卵巢癌患者的生存时间有影响(P<0.05)。Cox回归模型分析显示化疗耐药对生存时间有显著性影响[相对危险度(RR)为8.635,P<0.001]。患者年龄、有无腹水、有无大网膜转移、临床分期、病理分级、组织类型对生存时间均无影响(P>0.05)。Kaplan-Meier生存曲线显示化疗敏感组的生存率明显优于耐药组(P<0.05)。结论:Sorcin可能与卵巢癌化疗耐药相关。Sorcin表达水平可能影响卵巢癌患者的生存时间。化疗耐药是卵巢癌患者预后不良的独立危险因素。

Objective:To investigate the correlation between soluble resistance-related calcium-binding protein(Sorcin)and chemoresistance or overall survival in patients with ovarian cancer.Methods:We detected the expression of Sorcin in 27 cases of chemoresistant ovarian cancer tissue and 37 cases of sensitive ovarian cancer tissue by immunohistochemistry,and analyzed the relationship between the protein and clinicopathological features or chemoresistance of ovarian cancer.Log-rank test was used to analyze the single factor impact on overall survival,Kaplan-Meier analysis was used to describe survival curve,and Cox proportional hazard model was used for multivariate analysis.Results:The immunoreactive scores for Sorcin in chemoresistant ovarian cancer tissues were higher than those in the sensitive ovarian cancer tissues(P<0.001).The levels of Sorcin inovarian cancer tissue did not show statistical significance with different ages,tumor stages,classifications,tissue types,degrees of ascites,omentums,and tumor metastases(P>0.05).The correlation between Sorcin and overall survival in resistant and sensitive ovarian cancer groups was not statistically significant(P>0.05),while there was a negative correlation between the expression of Sorcin and the overall survival of total cases(r=–0.326,P<0.05).Log-rank test showed that the drug resistance factor had a distinct impact on overall survival(P<0.001),and the Sorcin expression had an impact on overall survival(P<0.05).However,correlation between overall survival and the ages,ascites,omentum carcinoma,pathological types,pathological grade or FIGO staging was not significant(P>0.05).Cox proportional hazard model showed that drug resistance had a significant effect on overall survival(P<0.001),with a relative risk at 8.635,and the survival curve of the ovarian cancer sensitive group was obviously superior to that of ovarian cancer drug resistance group.Conclusion:Sorcin may be associated with drug resistance in ovarian cancer.The expression of Sorcin is correlated with the overall survival.The lower the Sorcin expression,the longer the survival time.Chemoresistance may act as an important independent prognostic factor for the poor prognosis for ovarian cancer.