紫杉醇调控Wnt/β-catenin信号通路抑制鼻咽癌细胞株HONE1增殖

Proliferation inhibition of paclitaxel on nasopharyngeal carcinoma cell line HONE1 and the regulation for Wnt/β-catenin signaling pathways

目的 研究紫杉醇对鼻咽癌细胞株HONE1的增殖抑制作用及对Wnt/β-catenin信号通路的调控。方法 低、中、高剂量实验组鼻咽癌HONE1细胞以10,20,30 mol·L^-1紫杉醇处理,对照组细胞以等量生理盐水处理,各组分别干预12,24,48 h。以噻唑蓝(MTT)法及平板克隆形成实验检测鼻咽癌HONE1细胞增殖,以Hoechst 33258染色观察鼻咽癌HONE1细胞细胞核形态及凋亡情况,HONE1细胞Wnt/β-catenin信号通路相关蛋白表达以蛋白质印迹法检测。结果 对照组及低、中、高剂量实验组鼻咽癌HONE1细胞克隆形成率分别为(66. 12±3. 08)%,(41. 93±3. 36)%,(30. 84±2. 97)%,(21. 03±3. 01)%,细胞凋亡率分别(3. 28±0. 83)%,(13. 56±2. 14)%,(27. 01±2. 64)%,(34. 79±2. 52)%,Wnt4蛋白相对表达量分别为0. 76±0. 09,0. 67±0. 10,0. 18±0. 04,0. 11±0. 03,β-catenin蛋白相对表达量分别为0. 94±0. 17,0. 78±0. 14,0. 35±0. 03,0. 21±0. 02,低、中、高剂量实验组与对照组比较,差异均有统计学意义(均P <0. 05)。各实验组间HONE1细胞增殖抑制率差异均有统计学意义(均P <0. 05)。结论 紫杉醇可通过抑制Wnt/β-catenin信号通路活化有效抑制鼻咽癌HONE1细胞生长,诱导其凋亡。

Objective To explore the proliferation inhibition effect of paclitaxel on nasopharyngeal carcinoma cell line HONE1 and the regulation for inositol phosphate-3-kinase/protein kinase/p53(PI3 K/AKT/p53)signaling pathways.Methods The nasopharyngeal carcinoma HONE1 cells were managed by 10,20,30 mol·L^-1 paclitaxel and acted as experimental-L/M/H groups,the cells in control group were managed by equivalent normal saline,each group was treated for 12,24,48 h.The proliferation of HONE1 cells was detected by MTT assay and tablet clone forming experiments;the nuclear shape and apoptosis of nasopharyngeal carcinoma HONE1 cells were detected by Hoeschest33258 nucleus staining;the related protein expression in PI3 K/AKT/p53 signal pathway of HONE1 cells were detected by Western Blot(WB).Results The clone forming rates in control group and experimental-L/M/H groups were(66.12±3.08)%,(41.93±3.36)%,(30.84±2.97)%,(21.03±3.01)%,apoptosis rates were(3.28±0.83)%,(13.56±2.14)%,(27.01±2.64)%,(34.79±2.52)%,the relative expression of Wnt4 protein were 0.76±0.09,0.67±0.10,0.18±0.04,0.11±0.03,the relative expression ofβ-catenin protein were0.94±0.17,0.78±0.14,0.35±0.03,0.21±0.02,there were significant difference between control group and experimental-L/M/H groups(all P<0.05).There were signifianct difference of proliferation inhibition rate of HONE1 cell between control group and experimental group(all P<0.05).Conclusion Paclitaxel can inhibit the nasopharyngeal carcinoma cell HONE1 proliferation,promote the apoptosis through the inhibiting of activation of Wnt/β-catenin signaling pathways.