改良型PRF与普通型PRF在脂肪移植应用中的实验对比研究

Application Comparison of A-PRF and PRF in Fat Transplantation

目的:探索改良型富血小板纤维蛋白(advance platetel-rich fribin,A-PRF)与普通型富血小板纤维蛋白(platetel-rich fribin,PRF)在脂肪移植中的应用效果,进而为临床应用提供指导。方法:将18只实验兔随机分成三组,实验组(A-PRF组):取兔背部脂肪组织,将2ml A-PRF+1ml颗粒脂肪移植到兔耳分离好的区域;阳性对照组(PRF组):兔耳移植区移入2ml PRF+1ml颗粒脂肪;阴性对照组(NS组):兔耳移植区移入2ml生理盐水+1ml颗粒脂肪。分别于4周、8周、12周取材进行大体观察、称重、HE染色与免疫染色来比较三组间脂肪成活差异。结果:各时间点,A-PRF组的脂肪存活率及新生毛细血管密度明显优于其他两组,PRF组结果优于NS组,且三组间比较差异有统计学意义(P<0.05)。结论:A-PRF在增加脂肪细胞成活率、减轻炎症反应及促进毛细血管新生方面优于普通型PRF,这为A-PRF的临床使用提供参考。

Objective To explore the application of advanced platelet-rich fibrin(A-PRF)and common platelet-rich fibrin(PRF)in fat transplantation in rabbit.Provide guidance for clinical applications.Methods Eighteen experimental rabbits were divided into three groups randdomly.The experimental group(A-PRF group):adipose tissue was taken,and 2ml A-PRF+1ml granule fat was transplanted into the isolated area of rabbit ears.The positive control group(PRF group):2ml PRF+1ml granule fat was transferred into the transplantation area.The negative control group(NS group):2ml physiological saline+1ml granular fat was transplanted.Gross observation,weighing,HE staining and immunostaining were performed at 4 weeks,8 weeks,and 12 weeks to compare the survival of fat between the three groups.Results At each observation time,the fat survival rate and neonatal capillary density of the A-PRF group were significantly better than the other two groups.The positive control group was superior to the negative control group,and the difference was statistically significant(P<0.05).Conclusion A-PRF is superior to common PRF in increasing the survival rate of adipocytes,reducing inflammation and promoting capillary angiogenesis,which provides a reference for the use of A-PRF in clinic.