寻常型银屑病中DUSP1和DUSP4的表达

The expression of DUSP1 and DUSP4 in psoriasis vulgaris

目的:检测DUSP1和DUSP4在寻常型银屑病患者皮损的表达水平。方法:Real-time PCR和Western Blot分别检测18例寻常型银屑病皮肤组织和15名正常对照皮肤组织中DUSP1和DUSP4 mRNA及相关蛋白的表达情况。结果:银屑病组DUSP1 mRNA和蛋白的相对表达量分别为0.0939±0.0048和0.5165±0.0254,低于正常对照组的0.2486±0.0060和1.2432±0.3260,差异有统计学意义(P s<0.05)。银屑病组DUSP4 mRNA和蛋白的相对表达量为0.3718±0.0370和0.8018±0.0770,与正常对照组比较(0.3645±0.0286和1.3677±0.2287),差异无统计学意义(P s>0.05)。结论:DUSP1表达水平降低可能与寻常型银屑病发生发展有关。

Objective:To detect the expression level of DUSP1 and DUSP4 in the lesions of psoriasis vulgaris.Methods:The mRNA and protein level of DUSP1 and DUSP4 from 18 psoriasis lesions and 15 normal skin were detected by real-time PCR and Western blot respectively.Results:The levels of mRNA and protein of DUSP1 from psoriasis lesions were(0.0939±0.0048)and(0.5165±0.0254),which were lower than those in the normal skin(0.2486±0.0060 and 1.2432±0.3260),with significant differences(P s<0.05).There were no significant differences of mRNA and protein of DUSP1 in the psoriasis lesions(0.3718±0.0370)and(0.8018±0.0770)and normal skin(0.3645±0.0286)and(1.3677±0.2287)(P s>0.05).Conclusion:The level of DUSP1 in the psoriasis lesions decreases which may be related to the pathogenesis and development of psoriasis vulgaris.