SAA1在狼疮小鼠肾脏中的表达及其对小鼠肾小管上皮细胞EMT的作用

Expression of SAA1 in kidney of lupus mice and its effect on EMT of mRTECs

目的:探讨SAA1在狼疮小鼠肾脏中的表达及其对肾脏纤维化的影响。方法:选取自发性狼疮肾炎小鼠(LN小鼠)与阴性对照的C57/BL小鼠用于体内实验,应用Masson染色检测其肾脏组织纤维化程度,应用Real-time PCR、Western blot和免疫组化技术检测组织中SAA1以及相关EMT指标的表达情况;体外实验中,将mRTEC细胞应用不同处理后,分为空白对照组、LPS处理组、LPS+Si-NC和LPS+Si-SAA1组,应用Western blot和免疫细胞荧光检测相关蛋白表达。结果:LN小鼠肾脏发生明显纤维化,SAA1表达较正常组明显升高;LPS能诱导mRTECs中SAA1、FN和α-SMA的表达,并降低E-Ca蛋白水平,而同时沉默SAA1能有效减缓LPS这一作用。结论:SAA1在狼疮小鼠中表达明显升高,且能促进肾小管上皮细胞的EMT进程。

Objective:To investigate the expression of SAA1 in the kidney of lupus mice and its effect on renal fibrosis.Methods: Spontaneous lupus nephritis mice(LN mice) and C57/BL mice(negative control mice) were selected in vivo experiment.Masson staining was used to detect the degree of renal fibrosis.Real-time PCR,Western blot and immunohistochemical techniques were used to detect the expression of SAA1 and related EMT in tissues.MRTEC cells were divided into blank control group,LPS treatment group,LPS+Si-NC group and LPS+Si-SAA1 group after different treatments.Western blot and immunofluorescence were used to detect the expression of related proteins.Results: LN mice had obvious renal fibrosis,and the expression of SAA1 was significantly higher than that of the normal group.LPS could induce the expression of SAA1,FN and alpha-SMA in mRTECs,and reduce the level of E-Ca protein,while silencing SAA1 could effectively alleviate the effect of LPS.Conclusion: SAA1 expression in lupus mice is significantly increased and can promote the EMT process of renal tubular epithelial cells.