一测多评法测定肾康栓中的大黄蒽醌类成分

Determination of anthraquinones in Shenkang Suppositories by quantitative analysis multi-component with a single-marker

目的建立一测多评法测定肾康栓中大黄蒽醌类成分含量的HPLC法,并进行方法学考察。方法以大黄素为内标,建立芦荟大黄素、大黄酸、大黄酚和大黄素甲醚的相对校正因子。分别采用外标法和一测多评法测定肾康栓中5种大黄蒽醌类成分的含量。结果肾康栓中蒽醌类成分间的相对校正因子分别为f芦荟大黄素/大黄素=0.750,f大黄酸/大黄素=0.860,f大黄酚/大黄素=0.690,f大黄素甲醚/大黄素=0.930。采用一测多评法计算的含量与外标法实测值比较差异无统计学意义。结论一测多评法可用于测定肾康栓中5种大黄蒽醌类成分的含量。

Objective To establish an HPLC,quantitative analysis of multi-component with a single-marker(QAMS)to determine the contents of anthraquinones in Shenkang Suppositories.Methods Emodin was used as the internal reference substance,the relative correlation factors(RCF)of aloe-emodin,rhein,chrysophanol and physcion to emodin were established.The contents of these 5 anthraquinones were determined by the external method and QAMS.Results RCFs of aloe-emodin,rhein,chrysophanol and physcion to emodin were 0.750,0.860,0.690 and 0.930,respectively.There was no significant difference between QAMS method and external method.Conclusion QAMS can be used for determining the contents of 5 anthraquinones in Shenkang Suppositories.