水通道蛋白在干燥综合征中的表达及意义

Expressions and clinical significance of aquaporins in Sjogren’s syndrome

目的分析水通道蛋白(aquaporins,AQPs)在干燥综合征(Sjogren’s syndrome,SS)患者外周血中的表达水平和临床价值。方法收集临床确诊的23例SS患者为试验组,分别由其他自身免疫病(autoimmune diseases,AIDs)患者14例作为试验对照组和由来自体检中心20例健康人作为健康对照组。采用酶联免疫吸附法(ELISA)检测外周血AQP5和AQP8的表达。结果SS组AQP5水平比健康对照组表达增加(Z=1.826,P=0.041),AQP8表达水平差异无统计学意义(P>0.05)。SS组与试验对照组比较,AQP5和AQP8表达水平差异均无统计学意义(P>0.05)。SS患者外周血AQP5和AQP8的表达水平之间存在相关性(r=0.991,P<0.01)。SS患者外周血AQP5(r=0.4805,P=0.032)、AQP8(r=0.5357,P=0.0149)水平与外周血IgM具有相关性,二者与IgG、IgA、ESR、CRP、RF以及白细胞、血红蛋白和血小板之间无相关性(P>0.05);按照有无间质性肺疾病和有无血液系统受累、抗SSB抗体阴性和阳性以及唇腺病理分级对SS患者进行亚组分析,差异均无统计学意义(P>0.05)。结论AQP5在SS患者较健康人群表达增高,可能对于SS的诊断和发病机制研究具有一定价值。AQP8与AQP5的表达水平在SS患者具有相关性,其对于SS的意义有待进一步研究。

Objective To analyze the expression level and clinical value of aquaporins(AQPs)in peripheral blood of patients with Sjogren’s syndrome(SS).Methods Twenty-three SS patients of clinically confirmed were selected as experimental group.While 14 patients with autoimmune diseases(AIDs)were selected as experimental control group and 20 healthy subjects were selected as healthy control group.The expressions of AQP5 and AQP8 in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA).Results AQP5 in SS group was higher than that in healthy control group(Z=1.826,P=0.041),and there was no significant difference in the level of AQP8(P>0.05).There was no significant difference in the expression levels of AQP5 and AQP8 between SS group and experimental control group(P>0.05).The level of AQP5 was correlated with that of AQP8 in peripheral blood of SS patients(r=0.991,P<0.01).The levels of AQP5(r=0.4805,P=0.032)and AQP8(r=0.5357,P=0.0149)in peripheral blood of patients with SS correlated with peripheral blood IgM.And neither had relationship with IgG,IgA,ESR,CRP,RF and leukocytes,hemoglobin and platelets(P>0.05).The SS patients were separated into different subgroups by with or without interstitial lung disease,with or without hematological involvement,anti-SSB antibody negative and positive,and salivary gland pathological grade,there were no significant differences in subgroup analysis of patients(P>0.05).Conclusion The expression of AQP5 was higher in SS patients than in healthy people,which may have certain value for the diagnosis and the studies for pathogenesis of SS.The expression level of AQP8 related to the level of AQP5 in SS patients.And their significance for SS need further study.