半番鸭不同组织Cu/Zn-SOD基因表达及酶活性研究

Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues

【目的】首次对半番鸭不同组织Cu/Zn-SOD基因表达、酶活性进行研究,以期了解半番鸭不同组织抗氧化特性,为深入研究半番鸭对环境因子的响应能力及相关分子机制提供参考。【方法】通过实时荧光定量法、黄嘌呤氧化酶法和硫代巴比妥酸法分别测定了半番鸭心脏、肝脏、脾脏、肺脏、肾脏、肌胃和胸肌中的Cu/Zn-SOD mRNA相对表达量、酶活力和MDA含量。【结果】Cu/Zn-SOD mRNA在胸肌中相对表达量最高,总体趋势为胸肌>肝脏>肺>心脏>脾脏>肾脏/肌胃。酶活力测定结果显示,肝脏和肾脏Cu/Zn-SOD酶活力显著高于胸肌和肌胃。半番鸭胸肌MDA含量显著高于肝脏、肌胃和肾脏,提示胸肌可能更易受到氧化应激损伤。相关分析结果显示Cu/Zn-SOD活力与MDA含量呈显著负相关,而抗氧化酶活力与mRNA表达水平相关性不显著。【结论】半番鸭Cu/Zn-SOD基因表达和酶活力具有组织特异性。不同组织Cu/Zn-SOD活力差异可能与Cu/Zn-SOD自身的生理功能以及组织代谢水平有关。

【Objective】Expressions of Cu/Zn-SOD gene and activities of the antioxidant enzyme in various organs of mule ducks were determined.【Method】The gene expression in the heart,liver,spleen,lung,kidney,gizzard and chest muscles,antioxidant enzyme activities,and MDA contents of mule duck were determined by the qRT-PCR,xanthine-xanthine oxidase,and thiobarbituric acid methods.【Result】The greatest mRNA expression of Cu/Zn-SOD gene was found in the chest muscle followed by the tissues in the order of liver>lung>heart>spleen>gizzard/kidney of the ducks.The activities of Cu/Zn-SOD enzyme in the liver and kidney were significantly higher than those in the breasts and gizzard.The higher MDA content in the breasts than in the liver,gizzard or kidney indicated a greater susceptibility of the chest muscle to damage by oxidative stress.There was no significant correlation observed between the antioxidant enzyme activity and the mRNA expression level in different organs.However,the Cu/Zn-SOD enzyme activity was inversely correlated to MDA content in the tissues.【Conclusion】The Cu/Zn-SOD mRNA expression and enzyme activity were organ-specific.The differences in the enzymatic activities might stem from the diverse physiological functions of the enzymes and/or the tissue metabolism per se.