细菌耐药性监测在临床微生物检验中的应用价值分析

Analysis on application value of surveillance of bacterial resistance in clinical microbial examination

目的探究细菌耐药性监测在临床微生物检验中的应用价值分析。方法随机抽取2013年11月~2016年11月在我院临床所收集的尿标本、血液标本、痰标本及分泌物标本中分离出的500例致病菌株实行细菌耐药性监测,在临床微生物检验中进行菌种分布情况和细菌耐药性检测结果分析。结果500例致病菌株中,其中革兰阳性菌为148株、革兰阴性菌为352株。其中凝固酶阴性葡萄球菌检出率最高,其次为铜绿假单胞菌、大肠埃希菌。变形杆菌对氨苄西林耐药性最高为92.09%,肠杆菌属对头孢唑林的耐药性最高为42.78%,金黄色葡萄球菌中MRSA对青霉素和氨苄西林耐药性均达到100%,凝固酶阴性葡萄球菌中MRCNS(甲氧西林凝固酶阴性葡萄球菌(MRCNS)及MSCNS对青霉素及氨苄西林耐药性分别为100.00%、100.00%、96.72%及89.89%,肠球菌属对头孢唑林的耐药性最高为32.48%。结论在细菌耐药性逐渐升高及多重耐药菌株生成的现状下,细菌耐药性监测在临床微生物检验中显得尤为重要,通过监测使得细菌耐药性变化形势被准确的掌握,进而给予患者适当适量的药物进行临床治疗,最大程度上减少抗生素的过度使用,具有较高的临床价值。

Objective To explore analysis on application value of Surveillance of bacterial resistance in clinical microbial examination.Methods 500 cases of pathogenic strains isolated from urine samples,blood samples,sputum samples and secretion samples collected from our hospitals from November 2013 to November 2016 were randomly selected and they were given surveillance of bacterial resistance.The distribution of strains and the results of surveillance of bacterial resistance were analyzed in clinical microbial examination.Results Among the 500 pathogenic strains,148 were Gram-positive bacteria and 352 were Gram-negative bacteria.The detection rate of coagulase negative staphylococcus was the highest,followed by pseudomonas aeruginosa and escherichia coli.The highest resistance of proteus to ampicillin was 92.09%,the resistance of enterobacter to cefazolin was 42.78%and the resistance of MRSA to penicillin and ampicillin in staphylococcus aureus was 100%.The resistance of MRCNS(Methicillin Resistant Coagulase-Negative Staphylococci(MRCNS))and MSCNS to penicillin and ampicillin in coagulase-negative staphylococci were 100.00%,100.00%,96.72%and 89.89%,respectively.The resistance of enterococcus to cefazolin was up to 32.48%.Conclusion In the current situation of increasing bacterial resistance and the emergence of multi-drug resistant strains,surveillance of bacterial resistance is particularly important in clinical microbial examination.Through monitoring,the change of bacterial resistance can be accurately mastered,and patients can be given appropriate and appropriate drugs for clinical treatment to minimize the overuse of antibiotics.It has high clinical values.