基于SDS-PAGE与荧光标记检测技术的黄淮麦区小麦品种(系)HMW-GS组成分析

HMW-GS Composition Analysis of Wheat Varieties(Lines) in Huanghuai Region Based on SDS-PAGE and Fluorescence Markers

利用SDS-PAGE和荧光标记检测技术同时对91份黄淮麦区小麦品种(系)的高分子质量麦谷蛋白亚基(HMW-GS)组成进行分析,以期为黄淮麦区小麦品质改良提供理论依据。SDS-PAGE检测结果表明,在Glu-1位点共出现14种亚基类型和28种亚基组合。Glu-A 1位点有3种亚基类型,分别是1(59.34%)、Null(37.36%)、2^*(3.30%);Glu-B1位点有5种亚基类型,分别是7+8(52.75%)、7+9(25.27%)、7(12.09%)、13+16(7.69%)、14+15(2.20%);Glu-D1位点有6种亚基类型,2+12(46.15%)、3+12(13.19%)、4+12(8.79%)、5+10(29.67%)、10(1.10%)和12(1.10%)。主要亚基组合类型为1/7+8/5+10(14.29%)、1/7+8/2+12(12.09%)、Null/7+8/2+12(12.09%)。荧光标记检测结果表明,供试材料中含有Ax 2^*、Dx2、Dx5、Dy10、Dy12的材料分别为3、42、27、28、63份,与SDS-PAGE检测结果相吻合。供试材料品质评分介于5.5~11.0分,平均评分为7.78分,亚基组合Null/13+16/5+10和1/13+16/2+12评分较高,均在10.0分及以上。聚类分析结果表明,供试材料可分为4类,其中第Ⅲ类集中了大部分强筋小麦材料。

The high molecular weight glutenin subunit(HMW-GS)composition of wheat varieties(lines)from Huanghuai region was analyzed by SDS-PAGE and fluorescence markers,so as to provide theoretical basis for quality improvement of wheat in Huanghuai region.The results of SDS-PAGE showed that in all 91 wheat varieties(lines),a total of 14 types of HMW-GS were detected,including three[Null(37.36%),2^*(3.30%),1(59.34%)]at Glu-A 1,five[7(12.09%),7+8(52.75%),7+9(25.27%),13+16(7.69%),14+15(2.20%)]at Glu-B 1 and six[2+12(46.15%),3+12(13.19%),4+12(8.79%),5+10(29.67%),10(1.10%)and 12(1.10%)]at Glu-D 1;the main combination types were 1/7+8/5+10(14.29%),1/7+8/2+12(12.09%),Null/7+8/2+12(12.09%).The results of fluorescence marker showed that there were 3,42,27,28,63 materials containing Ax 2^*,Dx2,Dx5,Dy10 and Dy 12,respectively,which was consistent with SDS-PAGE result.The quality score ranged from 5.5 to 11.0 with an average of 7.78,and the scores of Null/13+16/5+10 and 1/13+16/2+12 were higher,which were equal or greater than 10.0.The results of cluster analysis showed that 91 wheat varieties(lines)were divided into four classes,and classⅢhad the most of the strong gluten materials.