摘要
为综合评价公猪精液质量,研究公猪精液质量参数和精子形态参数的品种差异,并探讨公猪精液质量随年龄的变化规律,选取杜洛克、长白和大白公猪共114头,采用一般线性模型,以品种和年龄为固定因素对其精液质量参数、精子形态参数进行多变量分析。结果显示,杜洛克公猪的精子密度(619.20百万个/mL)显著(P<0.05)高于长白公猪(363.18百万个/mL)和大白公猪(336.92百万个/mL);杜洛克与大白公猪的精子活力和直线运动率均显著高于长白公猪(P<0.05);长白公猪精子正常形态率(92.95%)显著(P<0.05)高于杜洛克公猪(90.78%);1年龄的公猪精子密度(556.98百万个/mL)显著(P<0.05)高于2、3年龄(382.44百万个/mL、373.32百万个/mL);大白公猪精液的弯尾率、卷尾率均显著(P<0.05)低于杜洛克公猪和长白公猪,长白公猪的精子近端质滴率(2.28%)显著(P<0.05)低于杜洛克公猪(3.94%)和大白公猪(2.92%),3年龄的公猪精子近端质滴率显著(P<0.05)低于2年龄;在正常精子形态参数方面,杜洛克公猪和大白公猪精子的头部长度、尾巴长度均显著高于长白公猪(P<0.05),杜洛克公猪(2.96μm)和长白公猪(2.94μm)精子的头部宽度均显著(P<0.05)高于大白公猪(2.87μm),杜洛克、大白、长白公猪精子的头部周长依次降低且彼此间差异显著(P<0.05),长白公猪精子的头部面积/总精子长度显著(P<0.05)高于大白公猪,长白公猪的头部宽度/头部长度显著(P<0.05)高于杜洛克公猪和大白公猪,1、2年龄的公猪精子头部长度、头部周长、头部长度/总精子长度、头部长度/尾巴长度均显著(P<0.05)高于3年龄,1、2年龄的公猪精子尾巴长度/总精子长度和头部宽度/头部长度均显著(P<0.05)低于3年龄。综上,杜洛克公猪常规精液质量参数最优,其次为大白公猪和长白公猪,但其精子形态参数相比于长、大白公猪较劣。年龄因素对公猪精液质量影响不显著。
In order to comprehensively evaluate the quality of boar semen,we study the effect of breed and age on the semen quality and morphological parameters of sperm in boars.114 boars were selected and multivariate analysis was performed on routine semen quality parameters,abnormal morphological parameters of sperm,and normal morphological parameters of sperm using a general linear model with breed and age as fixed factors.Results showed that in the routine semen quality,sperm density of Duroc(619.20 million/mL)was significantly higher(P<0.05)than Landrace(363.18 million/mL)and Yorkshire(336.92 million/mL),the sperm vitality and linear motion rate of Duroc and Yorkshire were significantly(P<0.05)higher than Landrace.The normal form rate of Landrace boars sperm(92.95%)was significantly(P<0.05)higher than Duroc(90.78%),the sperm density of one year old boars(556.98 million/mL)was significantly(P<0.05)higher than that of two and three years old boars(382.44 million/mL,373.32 million/mL).In terms of anomalous sperm morphological parameters,the bent tail rate and the curled tail rate of Yorkshire were significantly(P<0.05)lower than Duroc and Landrace,the proximal droplets rate of Landrace boars sperm(2.28%)was significantly(P<0.05)lower than Duroc(3.94%)and Yorkshire(2.92%),the proximal droplets rate of three years old boars was significantly(P<0.05)lower than two years old boars.In terms of normal sperm morphology parameters,the head and tail length from boar of Duroc and Yorkshire were significantly(P<0.05)higher than Landrace,the head width of Duroc(2.96μm)and Landrace(2.94μm)was significantly higher(P<0.05)than Yorkshire(2.87μm),the head circumference of Duroc,Yorkshire and Landrace decreased in turn and was significantly different(P<0.05),the head area/total sperm length of Landrace was significantly(P<0.05)higher than Yorkshire,the head width/head length of Landrace boar was significantly higher(P<0.05)than that of Duroc and Yorkshire,the sperm head length,head circumference,head length/total sperm length,head length/tail length of boars of one and two years old were significantly higher(P<0.05)than three years old,the tail length/total sperm length and head width/head length of boars of one and two years old were significantly(P<0.05)lower than three years old.Results indicated that in terms of conventional semen quality parameters,Duroc was the best,followed by Yorkshire and Landrace,but the morphology parameters of Duroc was at a disadvantage compared to Landrace and Yorkshire.The factor of age had little effect on boar semen quality.
作者
赵云翔
方程
朱琳
高广雄
叶礼
张从林
李智丽
ZHAO Yunxiang;FANG Cheng;ZHU Lin;GAO Guangxiong;YE Li;ZHANG Conglin;LI Zhili(College of Life Science and Engineering,Foshan University,Foshan 528225,China;Guangxi Yangxiang Co.,Ltd.,Guigang 537100,China;Guangxi Xiubo Technology Co.,Ltd.,Guigang 537100,China)
出处
《河南农业科学》
北大核心
2019年第11期157-162,共6页
Journal of Henan Agricultural Sciences
基金
广东省科技创新战略专项(2018B020203002)