BrVIN3.1互作蛋白BrZAT12在大白菜春化途径中行使功能初探

Preliminary Studies on The Function of BrZAT12,a Protein Interacted with BrV IN3.1,during Flowering Time in Chinese Cabbage

BrVIN3.1在春化过程中通过调控BrFLC1的表观修饰状态,进而调控其表达控制大白菜花期。本试验以BrVIN3.1(Bra020445)为诱饵,进行酵母cDNA文库筛选,从中筛选到它的互作蛋白BrZAT12(Bra006691);进一步进行酵母双杂一对一验证,证明BrZAT12能够与BrVIN3.1互作。采用低温处理不同花期的大白菜材料,进行表达模式分析,发现BrZAT12的表达模式在抽薹性状不同的大白菜材料中存在差异,在易抽薹材料中的表达量明显高于耐抽薹材料,且在耐抽薹材料中变化幅度较小,同时BrZAT12的表达模式与BrVIN3.1相关。推测BrZAT12可能在大白菜春化调控的开花途径中行使功能。

During flowering time process,BrVIN3.1 regulates by mediating the epigenetic states of BrFLC1,regulated its expression then controlled the flowering time of Chinese cabbage.Taking BrVIN3.1(Bra020445)as bait,the paper carried out screen selection on yeast cDNA library and obtained BrVIN3.1’s interaction protein and BrZAT12(Bra006691).Yeast-two-hybrid verification was conducted.The results showed that they did interact in vivo.Chinese cabbage materials at different flowering times were treated by refrigeration.Expression pattern was analyzed.The results showed that BrZAT12 expression patterns were different in Chinese cabbage materials at different bolting times.The expression quantities of easy bolting materials were higher than those of tolerant bolting materials,which had lesser amplitude of variation.In summary,the paper speculated that BrZAT12 may function in flowering pathway by regulating the expression of BrVIN3.1 in Chinese cabbage.