隐甲藻生产二十二碳六烯酸(DHA)的研究进展

Docosahexaenoicacid Produced by Crypthecodinium cohnii: Research Progress

二十二碳六烯酸(docosahexaenoicacid,DHA)是具有重要生理功能而人体自身无法合成的一类多不饱和脂肪酸,而隐甲藻(Crypthecodinium cohnii)是生产二十二碳六烯酸的理想原料之一。为了将来开展隐甲藻规模化生产DHA研究,综合分析了隐甲藻种质、DHA生物合成途径、培养条件对DHA积累的影响等方面的问题,包括:(1)组成隐甲藻的同胞体DHA产量不同,可利用诱变的方式筛选突变体获得新的优良品系;(2)隐甲藻DHA的生物合成与其他脂肪酸的生物合成不同,对其合成途径进行了推测,可能为PKS途径;(3)环境条件,包括温度、光照、pH值、溶氧量、接种量、消泡处理等,以及培养基组成,包括碳源、氮源、无机盐及微量元素等培养条件均会影响藻体中DHA的含量。最后提出了对隐甲藻生产DHA的研究展望。

Docosahexaenoicacid(DHA)is a class of polyunsaturated fatty acid with important physiological functions which the body could not synthetise.Crypthecodinium cohnii is one of the ideal raw materials for producing DHA.In order to study the large-scale production of DHA by Crypthecodinium cohnii in the future,we comprehensively analyze Crypthecodinium cohnii germplasm,DHA biosynthesis way,and the effect of culture conditions on DHA accumulation,including:(1)the DHA production of the siblings of the cryptophyta is different,and the mutants can be screened to obtain new excellent lines by mutagenesis;(2)the biosynthesis of DHA is different from the biosynthesis of other fatty acids,the synthetic route is speculated,possibly is PKS pathway;(3)environmental conditions,including temperature,light,pH value,dissolved oxygen,inoculum,defoaming,etc.;and medium composition,including carbon source,nitrogen source,culture conditions such as inorganic salts and trace elements could affect the content of DHA in algae.At last we put forward the research prospect of DHA produced by Crypthecodinium cohnii.