长链非编码RNA FENDRR通过靶基因微RNA-181b-5p调控口腔鳞癌细胞增殖、侵袭迁移的分子机制

Molecular mechanism of long-chain non-coding RNA FENDRR regulating proliferation,invasion and migration of oral squamous cell carcinoma cells by target gene miR-181b-5p

目的探讨长链非编码RNA(lncRNA)FENDRR对口腔鳞癌细胞增殖、侵袭迁移的影响及其可能作用机制.方法以人口腔鳞癌细胞SCC15和人正常口腔上皮细胞HOEC为研究对象,RT-qPCR检测细胞中FENDRR和miR-181b-5p表达,MTT法、Transwell小室法分别检测过表达FENDRR、抑制miR-181b-5p、同时过表达FENDRR和miR-181b-5p对口腔鳞癌细胞增殖、迁移侵袭的影响,双荧光素酶报告基因实验检测FENDRR是否靶向miR-181b-5p.结果与正常口腔上皮细胞相比,口腔鳞癌细胞中FENDRR表达明显下调(P<0.05),miR-181b-5p表达明显上调(P<0.05);过表达FENDRR明显抑制口腔鳞癌细胞中miR-181b-5p表达,而抑制FENDRR后miR-181b-5p表达明显升高,双荧光素酶报告基因实验结果证实FENDRR靶向miR-181b-5p;过表达FENDRR、抑制miR-181b-5p均明显抑制口腔鳞癌细胞增殖、迁移和侵袭,而过表达miR-181b-5p则可逆转FENDRR过表达对口腔鳞癌细胞增殖、迁移和侵袭的抑制作用.结论FENDRR具有抑制口腔鳞癌细胞增殖、侵袭迁移的作用,其机制可能与靶向抑制miR-181b-5p表达有关.

Objective To investigate the effect of long-chain non-coding RNA(lncRNA)FENDRR on the proliferation,invasion and migration of oral squamous cell carcinoma cells and its potential mechanism.Methods Human oral squamous cell carcinoma cell line SCC15 and human normal oral epithelial cell line HOEC were included as the subjects in the study.The expression of FENDRR and miR-181b-5p were determined by RT-qPCR.MTT assay and Transwell chamber assay were used to examine the overexpression of FENDRR and inhibition of miR-181b-5p,respectively.The effects of overexpression of FENDRR and inhibition of miR-181b-5p on the proliferation,migration and invasion of oral squamous cell carcinoma cells were determined.Dual luciferase reporter assay was used to determine whether FENDRR targeted miR-181b-5p.Results Compared with normal oral epithelial cells,the expression level of FENDRR was significantly down-regulated in oral squamous cell carcinoma cells(P<0.05),whereas the expression level of miR-181b-5p was significantly up-regulated(P<0.05).Overexpression of FENDRR significantly inhibited the expression level of miR-181b-5p in oral squamous cell carcinoma cells,whereas the expression level of miR-181b-5p significantly increased after FENDRR inhibition.The findings of dual luciferase reporter gene assay identified that FENDRR targeted miR-181b-5p.Overexpression of FENDRR and inhibition of miR-181b-5p significantly inhibited the proliferation,migration and invasion of oral squamous cell carcinoma cells,whereas overexpression of miR-181b-5p reversed the inhibition of FENDRR overexpression on proliferation,migration and invasion of oral squamous cell carcinoma cells.Conclusion FENDRR inhibits the proliferation,invasion and migration of oral squamous cell carcinoma cells,and its mechanism may be related to the targeted inhibition of miR-181b-5p expression.