地桃花质量标准的研究

Quality standard for Urena lobata

目的建立地桃花Urena lobata L.的质量标准。方法对地桃花形态、显微特征进行鉴定后,TLC法定性鉴别银锻苷,《中国药典》方法测定水分、总灰分、浸出物含有量,HPLC法测定银锻苷含有量。结果 TLC斑点清晰,重复性好;银锻苷在0.476 4~238.2μg/mL范围内线性关系良好(r=1.000 0),平均加样回收率为99.0%,RSD为2.28%。12批样品水分、总灰分、浸出物含有量范围分别为11.4%~14.0%、6.2%~12.6%、4.4%~9.6%。结论该方法稳定可靠,可用于地桃花的质量控制。

AIM To establish the quality standard for Urena lobata L..METHODS The morphological and microscopic characteristics of U. lobata were identified, after which TLC was used for the qualitative identification of tiliroside, Chinese Pharmacopoeia method was applied to determining the contents of water, total ash and extract, HPLC was adopted in the content determination of tiliroside.RESULTS The TLC spots were clear and reproducible. Tiliroside showed a good linear relationship within the range of 0.476 4-238.2 μg/mL(r=1.000 0), whose average recovery was 99.0% with the RSD of 2.28%. In twelve batches of samples, the content ranges of water, total ash and extract were 11.4%-14.0%, 6.2%-12.6% and 4.4%-9.6%, respectively.CONCLUSION This stable and reliable method can be used for the quality control of U. lobata.