HPLC法同时测定两粤黄檀中6种成分

Simultaneous determination of six constituents in Dalbergia benthamii by HPLC

目的建立HPLC法同时测定两粤黄檀中甘草酸、土甘草A、4′-羟基-6-羟甲基-5,7-二甲氧基异黄酮、大鱼藤树素甲醚、大鱼藤树酸甲酯、甘草次酸的含有量。方法两粤黄檀氯仿提取物的分析采用Waters-symmetry C18色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-乙腈-0.2%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长256 nm。结果甘草酸、土甘草A、4′-羟基-6-羟甲基-5,7-二甲氧基异黄酮、大鱼藤树素甲醚、大鱼藤树酸甲酯、甘草次酸分别在0.07~2.50(r=0.999 2)、0.03~1.15(r=0.999 2)、0.02~0.55(r=0.999 3)、0.01~0.26(r=0.999 8)、0.01~0.25(r=0.999 6)、0.01~0.36(r=0.999 0)mg/mL范围内线性关系良好,平均加样回收率分别为100.7%、101.5%、101.0%、97.4%、96.4%、100.5%,RSD分别为2.1%、2.1%、1.8%、2.0%、1.5%、2.1%。结论该方法准确稳定,重复性好,可用于两粤黄檀的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of glycyrrhizic acid, 3-phenylcoumarin robustic acid, 4′-hydroxy-6-hydroxymethyl-5,7-dimethoxy isoflavone, robustin methyl ether, methyl robustate, glycyrrhetinic acid in Dalbergia benthamii Prain.. METHODS The analysis of chloroform extract of D. benthamii was performed on a 30 ℃ thermostatic Waters-Symmetry C18(4.6 mm×250 mm,5 μm), with the mobile phase comprising of methanol-acetonitrile-0.2% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 256 nm.RESULTS The glycyrrhizic acid, 3-phenylcoumarin robustic acid, 4′-hydroxy-6-hydroxymethyl-5,7-dimethoxy isoflavone, robustin methyl ether, methyl robustate, glycyrrhetinic acid showed good linear relationships within the ranges of 0.07-2.50(r=0.999 2)、0.03-1.15(r=0.999 2)、0.02-0.55(r=0.999 3)、0.01-0.26(r=0.999 8)、0.01-0.25(r=0.999 6)、0.01-0.36(r=0.999 0)mg/mL,whose average recoveries were 100.7%、101.5%、101.0%、97.4%、96.4%、100.5% with the RSDs of 2.1%、2.1%、1.8%、2.0%、1.5%、2.1%,respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of D. benthamii.