3种竹叶抗氧化有效成分分析

Analysis of antioxidant effective components in three kinds of bamboo leaves

目的研究3种竹叶中有效成分含有量与其提取物抗氧化活性差异的相关性及其抗氧化活性基础物质。方法采用HPLC法分别测定了7批淡竹叶、10批苦竹叶、9批竹叶中7种成分的含有量;DPPH法、ABTS法及FRAP法测定其抗氧化活性。以清除DPPH自由基的能力评价3种竹叶提取物的抗氧化活性,并利用多元相关与回归分析方法探讨各成分含有量与提取物活性的关系。结果淡竹叶的抗氧化活性最弱,且与苦竹叶、竹叶比较均具有显著性差异(P<0.05)。芹菜素、异荭草苷、荭草苷含有量分别与淡竹叶、苦竹叶、竹叶抗氧化活性呈显著性正相关(P<0.05)。回归分析结果与相关性分析结果基本一致。结论芹菜素、异荭草苷、荭草苷分别是淡竹叶、苦竹叶、竹叶中的主要抗氧化有效成分。

AIM To study the functional antioxidant compounds in three kinds of bamboo leaves and the correlations between the content of effective components and antioxidant activities of the extracts.METHODS The contents of seven components in seven batches of Lophatherum gracile Brongn.,ten batches of Pleioblastus amarus(Keng) Keng f.and nine batches of Phyllostachys nigra(Lodd.) Munro var:henonis(Mitf.) Stapf ex Rendle leaves were determined by HPLC,and DPPH,ABTS,FRAP assay were used to evaluate their antioxidant capacity.The antioxidant activities of extracts were evaluated by the capability of scavenging DPPH free radical, and the relationships between the contents of compounds and the activities of extracts were explored by multiple correlation and regression analysis.RESULTS The antioxidant activities of L. gracile leaves were the weakest,and they were significantly different from P. amarus and P. nigra(P<0.05).The contents of apigenin,isoorientin,and orientin were significantly positively correlated with antioxidant activities of L. gracile,P. Amarus and P. nigra leaves(P<0.05), respectively.Stepwise regression analysis results were the same as bivariate correlation analysis results.CONCLUSION Apigenin,isoorientin,and orientin are the main effective components in L. gracile,P. Amarus, and P. nigra leaves, respectively.