糖尿病患者口腔种植体周围龈沟液中CCL2、CCL4和CXCL8表达及意义

Expressions and significance of CCL2,CCL4 and CXCL8 in oral peri-implant gingival crevicular fluid in diabetic patients

目的探讨糖尿病患者口腔种植体周围龈沟液中趋化因子(C-C基序)配体2(CCL2)、趋化因子(C-C基序)配体4(CCL4)和趋化因子(C-X-C基序)配体8(CXCL8)的表达及其对糖尿病患者种植体的影响。方法选取接受口腔种植体修复手术的糖尿病患者48例(48枚种植体),根据是否发生炎症分为糖尿病健康种植体27枚(A)组和糖尿病炎症种植体21枚(B)组,另纳入健康者健康种植体30枚作为对照(C)组。采用ELISA检测龈沟液中CCL2、CCL4和CXCL8表达,分析B组相关指标之间的相关性和影响糖尿病患者种植体炎症发生的危险因素。结果A组探诊深度、出血指数、CCL2、CCL4和CXCL8表达水平均与C组相仿(P>0.05);但B组上述指标均高于C组(P<0.05)。B组龈沟液中CCL2与CXCL8水平、出血指数均呈正相关(P<0.05),CCL4与CXCL8水平、探诊深度均呈正相关(P<0.05),CXCL8与探诊深度、出血指数均呈正相关(P<0.05)。CCL2和CXCL8高水平是影响糖尿病患者口腔种植体炎症发生的危险因素(P<0.05)。结论糖尿病患者口腔炎症种植体龈沟液中的CCL2、CCL4和CXCL8呈高表达,可能与糖尿病患者口腔种植体炎症的发生有关。

Objective To investigate the expressions of chemokine ligand 2(CCL2),chemokine ligand 4(CCL4) and chemokine ligand 8(CXCL8) in gingival crevicular fluid around the oral implants and their effects on the implants in diabetic patients.Methods Forty-eight patients(48 implants) with diabetes mellitus underwent oral implant repair were divided into two groups.The patients in group A(27 cases) were without inflammation around the oral implant and those in group B(21 cases) were with inflammation around the oral implant.Thirty healthy persons underwent oral implant without inflammation around the oral implant were taken as the controls(group C).The expressions of CCL2,CCL4 and CXCL8 in gingival crevicular fluid were detected by ELISA.The correlation among the relevant indicators and the risk factors for implant inflammation in group B were analyzed.Results The levels of probing depth,hemorrhage index,and the expressions of CCL2,CCL4 and CXCL8 in group A were similar to those in group C(P>0.05),which were significantly higher in group B than those in group C(P<0.05).CCL2 in gingival crevicular fluid of diabetic inflammatory implants was positively correlated with CXCL8 level and hemorrhage index(P<0.05), CCL4 was positively correlated with CXCL8 and probing depth(P<0.05),The expression level of CXCL8 in group B was positively correlated with probing depth and bleeding index(P<0.05).The high levels of CCL2 and CXCL8 expressions were the risk factors for peri-implant inflammation in diabetic patients(P<0.05).Conclusion The CCL2,CCL4 and CXCL8 in gingival crevicular fluid are highly expressed in diabetic patients with inflammation around the oral implant,which may be related to the occurrence of the inflammation.