DNA polβ基因对乳癌细胞MCF-7增殖及侵袭影响

EFFECTS OF THE DNA POLβ GENE ON PROLIFERATION AND INVASION OF BREAST CANCER MCF-7 CELLS

目的探究RNA干扰技术沉默DNA聚合酶β(DNA polβ)基因对人乳癌细胞的增殖、侵袭的影响。方法构建3种DNA polβ-siRNA质粒,转染人乳癌细胞(MCF-7),选择最优DNA polβ-siRNA质粒进行后续实验。应用荧光定量PCR和Western blot检测质粒转染效率;噻唑蓝(MTT)方法检测DNA polβ-siRNA对细胞增殖能力的影响;Transwell检测敲降DNA polβ基因对MCF-7细胞侵袭能力的影响。结果DNA polβ-siRNA不仅能够抑制MCF-7细胞中DNA polβ的表达,而且还能抑制MCF-7细胞的增殖(F=5.8、8.6,P<0.05)和侵袭能力(F=5.2,P<0.05)。结论DNA polβ-siRNA可有效抑制MCF-7细胞的增殖和侵袭。

Objective To explore the effects of silencing the DNA polymeraseβ(polβ)gene by RNA interference technology on the proliferation and invasion of human breast cancer cells.Methods Three DNA polβ-siRNA plasmids were constructed and then transfected into human breast cancer MCF-7 cells.The optimal DNA polβ-siRNA plasmid was selected for subsequent experiments.The efficiency of plasmid transfection was determined by real-time PCR and Western blot.The effect of the DNA polβ-siRNA on cell proliferation was measured by MTT assay.The effect of knockdown of the DNA polβgene on cell invasion was measured by Transwell assay.Results The DNA polβ-siRNA significantly inhibited the expression of DNA polβin MCF-7 cells.Furthermore,the DNA polβ-siRNA significantly inhibited the proliferation(F=5.8,8.6;P<0.05)and invasion(F=5.2,P<0.05)of MCF-7 cells.Conclusion The DNA polβ-siRNA can effectively inhibit the proliferation and invasion of MCF-7 cells.