壮族与汉族颈动脉粥样硬化患者的血清同型半胱氨酸水平及亚甲基四氢叶酸还原酶基因多态性

Serum homocysteine level and gene polymorphism of methylenetetrahydrofolate reductase in carotid atherosclerosis patients with Zhuang and Han nationalities

目的比较壮族与汉族颈动脉粥样硬化(CAS)患者血清同型半胱氨酸(Hcy)水平及亚甲基四氢叶酸还原酶(MTHFR)C677T位点基因多态性的差异。方法将89例CAS患者,根据民族分为壮族组47例、汉族组42例。应用聚合酶联反应检测MTHFR C677T基因多态性,应用酶联免疫吸附法测定血清Hcy水平。结果壮族组的TT基因型及T等位基因频率均高于汉族组(均P<0.05)。壮族组的血清Hcy水平高于汉族组,但差异无统计学意义(P>0.05)。结论汉族与壮族CAS患者的MTHFR C677T位点基因多态性存在差异,但其血清Hcy水平不存在差异,CAS患者血清Hcy水平或不受代谢酶基因多态性的影响。

Objective To compare the serum homocysteine(Hcy) level and gene polymorphism at C677 T locus of methylenetetrahydrofolate reductase(MTHFR) between carotid atherosclerosis(CAS) patients with Zhuang and Han nationalities.Methods Eighty-nine patients with CAS were divided into Zhuang group(47 cases) and Han group(42 cases) according to nationality.The gene polymorphism at C677 T locus of MTHFR was detected by polymerase chain reaction,and serum Hcy level by enzyme-linked immunosorbent assay.Results The frequencies of TT genotype and T allele in the Zhuang group were higher than those in the Han group(all P<0.05).The serum Hcy level of the Zhuang group was higher than that of the Han group,but no statistically significant difference was found(P>0.05).Conclusion A difference in gene polymorphism at C677 T locus of MTHFR exists between CAS patients with Han and Zhuang nationalities,however,no difference in the serum Hcy level is observed,and the serum Hcy level of CAS patients may be not effected by the gene polymorphism of metabolic enzyme.