基于高通量测序的土壤中ermF基因宿主细菌多样性分析

Host Bacteria Diversity Analysis of ermF Gene in Farmland Soil Based on High-throughput Sequencing Analysis

为探究土壤环境中抗生素抗性基因宿主微生物的多样性,本研究以天津地区的农田土壤为研究对象,利用高通量测序技术对土样中大环内酯类抗性基因ermF的宿主细菌在不同分类水平的多样性进行了评估。土样中宿主细菌种类覆盖9个门,39个科,42个属。4个土样之间所共有的OTUs只占总OTUs数目的 0.5%,说明样品间宿主细菌群落的差异性显著,可能主要与采样地点不同有关。在所有土样中,ermF基因的优势宿主细菌在门的水平上均为拟杆菌门(Bacteroidetes,相对丰度50.3%~87.7%);同时检测到少量的变形菌门(Proteobacteria),放线菌门(Actinobacteria)和厚壁菌门(Firmicutes),占比均小于4.2%。土样中ermF优势宿主细菌在科水平上都为拟杆菌科(Bacteroidaceae,相对丰度50.3%~87.5%),在属水平上都为拟杆菌属(Bacteroides,相对丰度50.3%~87.5%),其中相对丰度大于0.1%的ermF的宿主菌属有14种。

To explore the host bacteria diversity of antibiotic resistance genes in soils, the high-throughput sequencing technique were used to evaluate the diversity of host bacteria of ermF, a macrolide resistance gene, at different taxonomic levels in farmland soils in Tianjin. The bacterial species in the samples covered 9 phyla, 39 families and 42 genera. The OTUs shared by the four soil samples only accounted for 0.5% of the total number of OTUs, which indicated that the difference of host bacterial community among the samples was significant, which might be related to the different sampling sites. At phylum level, the dominant host bacteria of ermF gene were Bacteroidetes(relative abundance 50.3%~87.7%) in all soil samples;a small number of Proteobacteria, Actinobacteria and Firmicutes were also detected, accounting for less than 4.2%. The dominant host bacteria of ermF were Bacteroidaceae(relative abundance 50.3% ~87.5%) at family level, and Bacteroides(relative abundance 50.3%~87.5%) at genus level. There were 14 genera of ermF host bacteria with relative abundances > 0.1%.