4-氯苯氧乙酸钠对小鼠的亚急性毒性及残留检测分析

Subacute toxicity and residue detection of sodium 4-chlorophenoxyacetate in mice

目的探讨毒豆芽中常用植物生长调节剂4-氯苯氧乙酸钠(sodium 4-chlorophenoxyacetate, 4-CPANa)对小鼠的亚急性毒性的影响以及其在小鼠机体的残留觃律。方法分别对小鼠按13.4、71.6、214.8 mg/kg(低、中、高)固定剂量连续灌胃28d,期间测定小鼠一般生理指标,结束后测定血液生化指标、脏器系数、病理变化,超高效液相色谱测定机体残留。结果与对照组相比,中、高剂量的4-CPANa对小鼠的一般生理指标、血清丙氨酸转氨酶(alanine aminotransferase, ALT)、天冬氨酸转氨酶(aspartate aminotransferase, AST)、尿素氮(blood urea nitrogen, BUN)等生化指标及肝脏、肾脏系数存在着显著性差异(P<0.05或P<0.01),造成中、高剂量组的小鼠肝脏、肾脏均出现组织病理学变化。小鼠机体中4-CPANa残留量均为:肾脏>肝脏>血液>心脏>脑>肌肉,且有剂量依赖关系。结论 4-CPANa的亚急性毒性会影响小鼠生长,损害肝脏、肾脏,28d灌胃损害的最低剂量(lowest observed adverse effect level,LOAEL)为71.6mg/kg,未观察到的有害作用剂量(no observed adverse effect level, NOAEL)为13.4 mg/kg。

Objective The aim of this study was to investigate subacute toxicity of Sodium 4-chlorophenoxyacetate(4-CPANa), one of plant growth regulator usually abused in the poison bean sprouts, in mice and its residue in their body. Methods Group low-dose, medial-dose, high-dose were gavaged with 4-CPANa solution of 13.4, 71.6, 214.8 mg/kg respectively daily for 28 days. Physiological index of mice were recorded during the experimental periods. Serum biochemical index, organ index and morphological examination of mice were carried out, and the 4-CPANa residue in a mouse’s body was analyzed by ultrahigh-performance liquid chromatography at the end of the experiment. Results Compared with the control group, the 4-CPANa exhibited significant influences(P<0.05 or P<0.01) on physiological index, organ index of mice include liver and kidney, serum biochemical index of mice include: Alanine aminotransferase(ALT), Aspartate aminotransferase(AST), Blood urea nitrogen(BUN) in the medial-dose and high-dose group compared with the control group. Furthermore, 4-CPANa also could result in significant histopathological changes in the liver and kidney of mice in medial-dose and high-dose group. The 4-CPANa residue in mice was observed as follows in decreasing order: kidney>liver>blood>heart>brain>muscle, and were dose-related. Conclusion The results suggested that 4-CPANa could influence on growth of mice and cause the lesion of liver and kidney in subacute toxicity. The lowest observed adverse effect level( LOAEL) of 4-CPANa was considered to be 71.6 mg/kg bw and no observed adverse effect level(NOAEL) to be 13.4 mg/kg bw.