调节性T细胞参与七氟烷预处理诱导的小鼠脑缺血保护

Regulatory T cells are involved in sevoflurane preconditioning-induced mouse brain protection against cerebral ischemia injury

目的明确调节性T细胞(Treg)是否参与七氟烷预处理诱导的小鼠脑缺血保护。方法 C57BL/6小鼠采用七氟烷预处理,并建立大脑中动脉阻塞模型,观察脑缺血再灌注后48 h脑梗死容积,并进行神经行为学评分,同时采用流式细胞术检测脾脏Treg比例;注射CD25中和抗体,再次观察七氟烷预处理后脑缺血再灌注损伤程度以及脾脏Treg比例。结果与对照组相比,七氟烷预处理可明显降低小鼠脑缺血再灌注损伤;同时,七氟烷预处理明显增加再灌注后48 h的Treg比例。与同型抗体相比, CD25中和抗体可逆转七氟烷预处理引起的Treg比例的增加;同时,这也逆转七氟烷预处理产生的小鼠脑缺血保护作用。结论 Treg参与七氟烷预处理诱导的小鼠脑缺血保护作用。

Objective To determine whether regulatory T cells(Tregs) are involved in sevoflurane preconditioning-induced brain protection against cerebral ischemia/reperfusion injury. Methods C57BL/6 mice were preconditioned with sevoflurane and then subjected to the middle cerebral artery occlusion modeling. The brain infarct volume and neurological score were assessed at 48 hours after cerebral reperfusion. Meanwhile, the proportion of Tregs in the spleen was analyzed by flow cytometry. Then, CD25 neutralizing antibody was administrated by intraperitoneal injection, following with the analysis of cerebral ischemia/reperfusion injury and the proportion of Tregs in the spleen after sevoflurane preconditioning. ResultsCompared with a control group, sevoflurane preconditioning markedly mitigated the cerebral ischemia/reperfusion injury in the mice including the infarct volume and neurological score. In the meantime, sevoflurane preconditioning significantly increased the proportion of Tregs in the spleen at 48 hours after cerebral reperfusion. Compared with the isotype antibody group, the CD25 neutralizing antibody reversed the increase of Tregs induced by sevoflurane preconditioning at 48 hours after reperfusion, which was also associated with the reversal of sevoflurane preconditioning-induced protectetion against cerebral ischemia/reperfusion injury. Conclusion Tregs are involved in sevoflurane preconditioning-induced cerebral protection against ischemia/reperfusion injury.