人参醇提物及沉淀对免疫低下小鼠的影响

Effect of Panax ginseng alcohol extract and precipitation on immunocompromised mice

目的:探讨人参醇提后的沉淀是否对免疫功能仍具有影响。方法:利用环磷酰胺免疫低下小鼠模型,观察人参水提物、醇提物和沉淀对小鼠免疫器官和血清免疫球蛋白G(Ig G)、白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)水平的影响。结果:空白组、模型组及给药各组小鼠胸腺、脾脏指数差异均无统计学意义(P>0.05);与空白组比较,模型组小鼠血清Ig G、IL-2、TNF-α水平均显著降低,差异有统计学意义(P<0.01);与模型组比较,阳性组和人参水提物组小鼠血清Ig G、TNF-α水平升高,差异有统计学意义(P<0.05,P<0.01),给药各组小鼠血清IL-2水平均较模型组升高,差异均有统计学意义(P<0.05)。结论:人参水提物能提高小鼠血清Ig G、IL-2、TNF-α水平,醇提物及沉淀能提高小鼠血清IL-2水平,说明沉淀物对免疫功能也有影响。

Objective:To investigate whether the precipitation after Panax ginseng alcohol extraction has a certain effect on immune function.Methods:An immunocompromised mouse model was established by cyclophosphamide to observe the effect of Panax ginseng water extract,Panax ginseng alcohol extract,and precipitation after extraction on immune organs and serum levels of immunoglobulin G(IgG),interleukin-2(IL-2),and tumor necrosis factor-α(TNF-α).Results:There were no significant differences in thymus index and spleen index between the blank group,the model group,and treatment groups(P>0.05).Compared with the blank group,the model group had significant reductions in the serum levels of IgG,IL-2,and TNF-α(P<0.01).Compared with themodel group,the positive group and the Panax ginseng water extract group had significant increases in the serum levels of IgG and TNF-α(P<0.05 or P<0.01),and compared with the model group,the treatment groups had a significant increase in the serum level of IL-2(P<0.05).Conclusion:Panax ginseng water extract can increase the serum levels of IgG,IL-2,and TNF-αin mice,and Panax ginseng alcohol extract and precipitation can increase the serum level of IL-2,suggesting that the precipitation also has a certain effect on immune function.