补肾清透方对肾虚型慢性荨麻疹小鼠模型细胞因子的影响

Effect of Bushen Qingtou prescription on cytokines in a mouse model of chronic urticaria with kidney deficiency

目的:研究补肾清透方(以下简称"补清方")对肾虚型慢性荨麻疹的作用机制。方法:选取昆明种雄性小鼠50只,随机分为空白对照组、模型组、氯雷他定组及补清方高、低剂量组,每组各10只。将除空白组外的40只小鼠与发情期雌鼠同笼,诱导其房事不节以制备肾虚模型,并采用注射卵白蛋白、氢氧化铝结合百日咳疫苗模拟荨麻疹模型。眼眶取血并分离血清,采用双抗夹心ELISA法测试血清免疫球蛋白E(IgE)、组胺(HIS);剥取小鼠主动脉,中性甲醛固定,采用免疫组织化学法检测血管壁组织中白细胞介素-4(IL-4)、细胞干扰素-γ(IFN-γ)的表达量。结果:与空白组比较,模型组血清IgE、HIS水平及血管壁组织中IL-4、IFN-γ的表达量均显著升高,差异有统计学意义(P<0.05)。与模型组比较,补清方高、低剂量组及氯雷他定组小鼠IgE、HIS、IL-4、IFN-γ含量明显降低,差异有统计学意义(P<0.05);3个用药组各个指标差异均无统计学意义(P>0.05)。结论:补清方可调整T细胞对相关细胞因子异常分泌以改善荨麻疹病情,是本病行之有效的治疗方剂。

Objective:To investigate the mechanism of action of Bushen Qingtou prescription in the treatment of chronic urticaria with kidney deficiency.Methods:A total of 50 male Kunming mice were randomly divided into blank control group,model group,loratadine group,and high-and low-dose Bushen Qingtou prescription groups,with 10 mice in each group.All mice except those in the blank control group were caged with female mice in estrus to induce excess of sexual intercourse,in order to establish a model of kidney deficiency,and they were given injection of ovalbumin,aluminum hydroxide,and pertussis vaccine to establish a model of urticaria.Orbital blood samples were collected,serum was isolated,and double-antibody sandwich ELISA was used to measure the serum levels of immunoglobulin E(IgE)and histamine(HIS);the aorta was collected and fixed by neutral formalin,and immunohistochemistry was used to measure the expression of interleukin-4(IL-4)and interferon-γ(IFN-γ)in vascular wall.Results:Compared with the blank control group,the model group had significant increases in the serum levels of IgE and HIS and the expression of IL-4 and IFN-γin vascular wall(P<0.05).Compared with the model group,the high-and low-dose Bushen Qingtou prescription groups and the loratadine group had significant reductions in IgE,HIS,IL-4,and IFN-γ(P<0.05),and there were no significant differences in the above indices between the three treatment groups(P>0.05).Conclusion:Bushen Qingtou prescription can improve urticaria by regulating the effect of T cells on the abnormal secretion of related cytokines and is thus an effective prescription for the treatment of this disease.