摘要
目的探讨叉头框D3蛋白(FOXD3)在胃癌组织中的表达及临床意义。方法选取118例胃癌患者的胃癌组织标本及对应的癌旁组织标本,采用免疫组化染色法及蛋白质印迹法(Western blot)检测两种标本中FOXD3蛋白的表达情况,并对胃癌组织中FOXD3蛋白表达与胃癌患者病灶直径、分化程度、TNM分期、浸润深度、淋巴结转移情况的关系进行分析。结果胃癌组织中FOXD3蛋白的阳性表达率和相对表达量均明显低于癌旁组织(P﹤0.01);TNM分期为Ⅲ~Ⅳ期、浸润深度为T3~4、有淋巴结转移、组织学分化程度为低分化胃癌患者胃癌组织中FOXD3蛋白的阳性表达率分别低于TNM分期为Ⅰ~Ⅱ期、浸润深度为T1~2、无淋巴结转移、组织学分化程度为高-中分化的患者(P﹤0.05)。结论FOXD3在胃癌组织中表达下调,可能与胃癌的发生发展有关。
Objective To investigate the expression of forkhead box D3(FOXD3)in gastric cancer tissues and the clinical significance.Method The specimens of gastric cancer tissues and corresponding paracancerous tissues of 118 patients with gastric cancer were selected.The expression of FOXD3 protein in the two specimens was detected by immunohistochemistry staining and Western blot.The relationship between FOXD3 protein expression and lesion diameter,degree of differentiation,TNM stage,depth of invasion and lymph node metastasis was analyzed.Result The positive expression rate and relative expression of FOXD3 protein in gastric cancer tissues were significantly lower than those in paracancerous tissues(P<0.01).The positive expression rates of FOXD3 protein in gastric cancer tissues of gastric cancer patients with TNM stageⅢ-Ⅳ,invasive depth T3-4,lymph node metastasis and poorly histological differentiation were lower than those with TNM stageⅠ-Ⅱ,invasive depth T1-2,no lymph node metastasis and high-medium histological differentiation(P<0.05).Conclusion The down-regulation of FOXD3 expression in gastric cancer tissues may be related to the development of tumors.
作者
唐思锋
张升波
王锋
李涛
TANG Sifeng;ZHANG Shengbo;WANG Feng;LI Tao(Department of Gastrointestinal Surgery,Ji’nan City People’s Hospital,Ji’nan 271100,Shandong,China)
出处
《癌症进展》
2019年第20期2423-2425,共3页
Oncology Progress
