两种铀配合物对生物分子的荧光标记性质研究

Fluorescence Labeling Properties of Two Uranium Complexes for Biomolecules

选用2种铀配合物分别与4种典型的生物分子多巴胺(DA)、抗坏血酸(AA)、尿酸(UA)和牛血清白蛋白(BSA)相互作用,采用荧光分光光度计进行荧光标记性质测试,研究铀配合物对4种生物分子的荧光标记性质。结果表明,4种生物分子对铀配合物的荧光发光产生了一定影响,在不同浓度梯度的生物分子中,浓度越大对铀配合物荧光强度的影响也越大;随着生物分子浓度的增大,出现了荧光猝灭现象,这2种铀配合物可以作为生物分子的荧光探针。为铀配合物在生物分子荧光标记研究方面的应用提供了一定的依据。

We selected two uranium complexes to interact with four typical biomolecules,dopamine(DA),ascorbic acid(AA),uric acid(UA),and bovine serum albumin(BSA),respectively,and studied the fluorescence labeling properties of uranium complexes with four biomolecules by fluorescence spectrophotometer.The results show that four biomolecules have certain effects on the fluorescence of uranium complexes.The higher the concentration of biomolecule is,the greater the effect of fluorescence intensity of uranium complexes will be.With the increasing of biomolecular concentration,fluorescence quenching of two uranium complexes occur.The uranium complexes can be used as fluorescent probes for biomolecules.The results can provide some effective research basis for the application of uranium complexes in biomolecular fluorescence labeling.