紫外分光光度法测定吴茱萸碱及吴茱萸次碱的溶解度及油水分配系数

Determination of Solubility and Oil-Water Partition Coefficient of Evodiamine and Rutaecarpine by UV Spectrophotometry

采用紫外分光光度法测定吴茱萸碱及吴茱萸次碱在甲醇中的溶解度及在不同pH值下的油水分配系数。结果表明,随着温度升高,吴茱萸碱、吴茱萸次碱在甲醇中的溶解度增大。吴茱萸碱在37℃时的溶解度较25℃时增大了近2.9倍;吴茱萸次碱在40℃时的溶解度较25℃时增大了近1.33倍。随PBS缓冲溶液pH值的增大,吴茱萸碱的油水分配系数整体呈增大趋势,吴茱萸次碱的油水分配系数呈先减小后增大趋势,吴茱萸碱和吴茱萸次碱的脂溶性逐渐升高。

We determined the solubilities of evodiamine and rutaecarpine in methanol,and the oil-water partition coefficients at different pH values by UV spectrophotometry.The results show that the solubilities of evodiamine and rutaecarpine in methanol increase with the increase of temperature.The solubility of evodiamine at 37 ℃ increases by nearly 2.9 times compared with that at 25 ℃.The solubility of rutaecarpine at 40 ℃ increases by nearly 1.33 times compared with that at 25 ℃.Moreover,the oil-water partition coefficient of evodiamine increases with the increase of pH value of PBS buffer solution,while the oil-water partition coefficient of rutaecarpine decreases first and then increases,and the lipophilicities of evodiamine and rutaecarpine increase gradually.