慢性乙型肝炎患者外周血IL-10,IFN-γ,IL-21及IL-22水平变化及临床意义

Changes of Serum Il-10、Ifn-γ、Il-21、and il-22 Levels in Patients with Chronic Hepatitis B and its Clinical Significance

目的探讨慢性乙型肝炎(CHB)患者外周血白介素-10(IL-10)、γ干扰素(IFN-γ)、白介素-21(IL-21)以及白介素-22(IL-22)的水平变化。方法选取2016年7月至2018年7月江苏省泰州市人民医院收治的48例CHB患者为研究对象,并选择20例健康人群作为正常对照。采用酶联免疫吸附(ELISA)法检测所有研究对象血清细胞因子IL-10、IFN-γ、IL-21以及IL-22水平,应用SPSS21.0软件进行统计学分析。结果CHB患者血清细胞因子IL-10、IL-21、IL-22水平与正常对照相比均存在明显差异,其中IL-10水平(340.72±129.23)pg/mL明显低于对照组(451.33±102.46)pg/mL,差异有统计学意义(t=20.823,P<0.001);IL-21和IL-22水平分别为(259.61±72.54)pg/mL和(242.88±95.55)pg/mL,明显高于对照组的(227.7±68.09)pg/mL、(199.21±56.22)pg/mL,差异有统计学意义(t=4.899,P=0.026和t=3.785,P=0.004);FIN-γ水平在病例组和对照组之间比较差异无统计学意义(t=1.082,P=0.114)。此外,4种血清细胞因子IL-10、IL-21、IL-22、FIN-γ水平与患者ALT均呈正相关,相关性系数分别为0.395、0.437、0.386、0.562,P<0.01。结论CHB患者血清细胞因子IL-10、IL-21、IL-22、FIN-γ水平可反应CHB患者炎症反应活动程度和疾病的严重程度,对于疾病的诊断和预后具有重要的临床意义。

Objective To investigate the changes of serum interleukin-10(IL-10),interferon-γ(IFN-γ),interleukin-21(IL-21)and interleukin-22(IL-22)levels in chronic hepatitis B(CHB)patients,analyze the correlation in the pathogenesis of CHB.Methods A total of 48 CHB patients were recruited at our hospital between July 2016 to July 2018,and 30 normal controls were set.Serum levels of IL-10、IL-21、IL-22 and IFN-γof two groups was detected by ELISA.The data were analyzed by statistical software SPSS 21.0.Results Serum levels of cytokines IL-10,IL-21 and IL-22 in CHB patients were significantly different from those in the normal control group.The levels of IL-10(340.72±129.23)pg/mLwere significantly lower than those in the control group(451.33±102.46)pg/mL,and the difference was statistically significant(t=20.823,P<0.001).Compared with control group,the levels of IL-21 and IL-22 were(259.61±72.54)pg/mL and(242.88±95.55)pg/mL,respectively,significantly higher higher than those of the control group(227.7±68.09)pg/mL and(199.21±56.22)pg/mL,with statistically significant(t=4.899,P=0.026 and t=3.785,P=0.004).The serum levels of IFN-γwas found no significant difference between the CHB patients and the control group(P>0.05).In addition,there was a positive correlation between the serum levels of IL-10、IL-21、IL-22、IFN-γand ALT(P<0.01),and the correlation coefficient is 0.395、0.437、0.386 and 0.562,respectively(P<0.01).Conclusion The levels of cytokines IL-10、IL-21、IL-22 and IFN-γcan reflect the degree of inflammatory response and the severity of the diseasein CHB patients,and it is of great significance for clinical diagnosis and prognosis judgement.