基质辅助激光解吸电离飞行时间质谱在结核病领域的应用进展

Progress on the application of matrix-assisted laser desorption ionization-time of flight mass spectrometry in the field of tuberculosis research

基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)具有高通量、高速度、自动化等优点,成为生物大分子研究的重要工具。其在临床研究领域取得显著进展,在结核病研究领域的作用也日益凸显。作者主要介绍了MALDI-TOF MS在分枝杆菌菌种鉴定、结核病耐药性研究、结核病血清标志物的筛选、结核病易感基因多态性检测和抗结核药物代谢研究方面的应用进展,以加强对MALDI-TOF MS在结核病研究领域的认识。

Matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS),which has the advantages of high throughput,high speed,automation,etc.,has become an important tool for bio-macromolecular research.It has made remarkable progress on clinical research and has become increasingly prominent in the field of tuberculosis research.This review mainly introduced the application of MALDI-TOF MS in the identification of mycobacterium,research of drug-resistance tuberculosis,screening of tuberculosis serum markers,detection of tuberculosis susceptibility gene polymorphisms and research of anti-tuberculosis drug metabolism in order to strengthen the understanding to MALDI-TOF MS in the field of tuberculosis research.