摘要
[Objectives]To optimize the extraction process of antioxidant active components from seeds of Firmiana platanifolia.[Methods]The seeds of F.platanifolia were taken as the research object.The primary samples of seeds of F.platanifolia were prepared following the procedure of selection,cleaning,shelling,drying and pulverization.The DPPH·(1,1-diphenyl-2-picrylhydrazyl)scavenging rate of the extract of seeds of F.platanifolia was used as an indicator,to conduct the single factor experiment for determining optimum single factors such as extraction solvent,extraction time,solid-to-liquid ratio,and extraction temperature.Then the experimental design was optimized by the response surface methodology.[Results]Single factor experiment showed that the optimal extraction solvent for the antioxidant active components of F.platanifolia seeds was ethyl acetate,the optimal extraction time was 50 min,the optimum solid-to-liquid ratio was 1∶6 g/mL,and the optimal extraction temperature was 60℃.The response surface optimization experiment showed that the optimal extraction process of the antioxidant active component extract of F.platanifolia seeds was:the extraction solvent was ethyl acetate,the extraction time was 45 min,the solid-to-liquid ratio was 1∶5 g/mL,and the extraction temperature was 55℃,which was basically consistent with the results of single factor experiment.Under the optimal extraction conditions,the DPPH·scavenging rate of ethyl acetate extract(200 mg/mL)of F.platanifolia seeds was 94.25%,which was equivalent to that of vitamin E(V E)solution(147.39 mg/L)dissolved with ethyl acetate.[Conclusions]The ethyl acetate extract of F.platanifolia seeds has a strong antioxidant capacity of eliminating DPPH·in vitro.
[Objectives] To optimize the extraction process of antioxidant active components from seeds of Firmiana platanifolia. [Methods] The seeds of F. platanifolia were taken as the research object. The primary samples of seeds of F. platanifolia were prepared following the procedure of selection, cleaning, shelling, drying and pulverization. The DPPH·(1,1-diphenyl-2-picrylhydrazyl) scavenging rate of the extract of seeds of F. platanifolia was used as an indicator, to conduct the single factor experiment for determining optimum single factors such as extraction solvent, extraction time, solid-to-liquid ratio, and extraction temperature. Then the experimental design was optimized by the response surface methodology. [Results] Single factor experiment showed that the optimal extraction solvent for the antioxidant active components of F. platanifolia seeds was ethyl acetate, the optimal extraction time was 50 min, the optimum solid-to-liquid ratio was 1∶6 g/mL, and the optimal extraction temperature was 60 ℃. The response surface optimization experiment showed that the optimal extraction process of the antioxidant active component extract of F. platanifolia seeds was: the extraction solvent was ethyl acetate, the extraction time was 45 min, the solid-to-liquid ratio was 1∶5 g/mL, and the extraction temperature was 55 ℃, which was basically consistent with the results of single factor experiment. Under the optimal extraction conditions, the DPPH· scavenging rate of ethyl acetate extract(200 mg/mL) of F. platanifolia seeds was 94.25%, which was equivalent to that of vitamin E(V_E) solution(147.39 mg/L) dissolved with ethyl acetate. [Conclusions] The ethyl acetate extract of F. platanifolia seeds has a strong antioxidant capacity of eliminating DPPH· in vitro.
基金
Supported by High Level Talent Foundation Project of Qingdao Agricultural University(663-1118006)
