枸杞多糖对离心过程中弱精精子氧化应激的作用研究

Effect of Lycium barbarum polysaccharides on oxidative stress in asthenospermia sperm during centrifugation process

目的探讨枸杞多糖对体外离心过程中弱精精子氧化应激的作用。方法按《世界卫生组织人类精液检查与处理实验手册》(2011年第五版)标准选取32份弱精症患者精子标本,每份标本各取1200μL,分为空白对照组、离心组和枸杞多糖组,各400μL。检测3组精液精子的前向运动精子百分率(PR)、存活率、正常形态、丙二醛(MDA)、活性氧(ROS)、DNA断裂指数(DFI)、高DNA着色性(HDS)、精子线粒体膜电位(MMP)情况。结果枸杞多糖精子PR和存活率高于离心组,但低于空白对照组,差异有统计学意义(P<0.05)。枸杞多糖组精子正常形态率与离心组无明显差异,但低于空白对照组(P<0.05)。枸杞多糖组DFI和HDS明显低于离心组,MMP高于离心组,差异有统计学意义(P<0.05)。枸杞多糖组MDA水平与空白对照组无差异,但低于离心组(P<0.05)。枸杞多糖组ROS阳性细胞率高于空白对照组,但低于离心组,差异有统计学意义(P<0.05)。结论一定浓度的枸杞多糖可以减弱弱精精子离心过程中过量的ROS对精子的氧化应激损害,从而提高精子线粒体活性,提高精子的PR和存活率,改善精子洗涤过程中的弱精精子功能和质量。

Objective To investigate the effect of Lycium barbarum polysaccharides(LBP)on anti-oxidative stress injury in the in vitro centrifugation process of asthenospermia sperm.Methods According to the standard in the fifth edition of the WHO Human Semen Examination and Processing Experiment Manual,32 samples of sperm from the patients with asthenospermia were selected,and 1200μL of sperm was taken from each sample,which were divided into the blank control group,centrifugal group and LBP group,400μL in each group.The sperm forward motility(PR),survival rate,normal morphology,malondialdehyde(MDA)level,reactive oxygen species(ROS),sperm DNA fragmentation index(DFI),HDS and sperm mitochondrial membrane potential(MMP)were detected in the three groups.Results The sperm PR value and sperm survival rate in the LBP group were higher than those in the centrifugal group,while lower than those in the blank control group,and the differences were statistically significant(P<0.05).There was no statistically significant difference in the normal morphology rate between the LBP group and the centrifugal group,but the normal morphology rate in the LBP group was lower than that in the blank control group(P<0.05).The DFI and HDS results all showed that in the LBP group were significantly lower than in the centrifugal group,while the MMP level in the LBP group was significantly higher than that in the centrifugal group with statistical difference(P<0.05).The MDA level had no difference between the LBP group and the blank control group,but the MDA level in the LBD group was lower than that in the centrifugal group(P<0.05).The positive cell rate of ROS in the LBP group was significantly higher than that in the blank control group,but lower than that in the centrifugal group,and the differences were statistically significant(P<0.05).Conclusion During the centrifugation process of asthenospermia sperm,adding a certain concentration of LBP can alleviate the oxidative stress damage to sperm caused by excessive ROS,thereby improve the sperm mitochondrial activity,enhance the sperm PR and viability of sperms,and improve the function and quality of asthenospermia sperm during sperm washing process.