甲状腺结节穿刺物中miRNA-221和miRNA-125的表达及意义

Expression and significance of miRNA-221 and miRNA-125 in thyroid nodule puncture samples

目的探讨甲状腺结节穿刺物中miRNA-221、miRNA-125的表达及意义。方法2016年6月至2018年8月收集青海大学附属医院肿瘤外科二科收治的138例经细针穿刺细胞学检查(FNAB)后仍不确定良恶性的甲状腺结节患者,采用TaqMan荧光探针检测甲状腺结节穿刺物中miRNA-221-3p、miRNA-125b-5p的表达。所有患者进行甲状腺结节切除手术,明确病理学诊断。结果138例甲状腺结节穿刺物中miRNA-221-3p、miRNA-125b-5p均有表达。miRNA-221-3p表达量良性组0.825,恶性组4.305;miRNA-125b-5p表达量良性组1.085,恶性组2.690;恶性组表达量较良性组均明显上调(P<0.01)。检测miRNA-221-3p、miRNA-125b-5p诊断恶性甲状腺结节的临界值分别为1.4450、1.8450,miRNA-221-3p或miRNA-125b-5p诊断恶性甲状腺结节的灵敏度分别为0.864、0.750,特异度分别为0.745、0.777,miRNA-221-3p联合miRNA-125b-5p诊断恶性甲状腺结节的灵敏度为0.705,特异度为0.904。结论miRNA-221-3p、miRNA-125b-5p的检测有助于评估甲状腺结节的良恶性。

Objective To explore the expression and significance of miRNA-221 and miRNA-125 in the puncture samples of thyroid nodules.Methods One hundred and thirty-eight patients with thyroid nodules who were still unclearly diagnosed by fine needle aspiration biopsies(FNAB)in the second department of oncology surgery of the Affiliated Hospital of Qinghai University from June 2016 to August 2018 were collected,and the expression levels of miRNA-221-3p and miRNA-125b-5p in fine needle aspiration samples of thyroid nodule were detected by adopting the TaqMan fluorescent probe.All patients conducted the resection operations of thyroid nodules for clearing the pathological diagnosis.The patients were divided into the benign group(n=94)and malignant groups(n=44),and the statistical analysis was performed by using the Mann-Whitney test.Results Both miRNA-221-3p and miRNA-125b-5p in fine needle aspiration samples were expressed.The expression level of miRNA-221-3p was 0.825 in the benign group and 4.305 in the malignant group,while the expression level of miRNA-125b-5p was 1.085 in the benign group and 2.690 in the malignant group.Compared with the benign group,the expression levels in the malignant group were significantly up-regulated(P<0.01).The cut-off values by testing miRNA-221-3p and miRNA-125b-5p to diagnose malignant thyroid nodule were 1.4450 and 1.8450 respectively.The sensitivities for diagnosing malignant thyroid nodule simply according to miRNA-221-3p and miRNA-125b-5p were 0.864 and 0.750 respectively,the specificity were 0.745 and 0.777 respectively,while the sensitivity and the specificity of miRNA-221-3p combined with miRNA-125b-5p for diagnosing malignant thyroid nodule were 0.705 and 0.904 respectively.Conclusion The test of miRNA-221-3p and miRNA-125b-5p is helpful for evaluating the benign or malignant nature of thyroid nodules.