应用神经突方向分散度和密度成像技术研究rs747302基因单核苷酸多态性对青春期儿童脑白质微结构的影响

Effect of DRD4 receptor -616 C/G polymorphism on white matter microstructure in normal adolescents, a MRI study using neurite orientation dispersion and density imaging technique

目的应用神经突方向分散度和密度成像技术(neurite orientation dispersionand densityimaging,NODDI)技术,探讨多巴胺D4受体基因(DRD4)616位点(rs747302)单核苷酸多态性(single nucleotide polymorphism, SNP)对正常青春期儿童白质微结构的影响。材料与方法该研究为前瞻性研究,通过rs747302基因型检测及NODDI扫描,获取2016年1月至2018年12月期间179例青春期儿童的基因型数据及磁共振NODDI扫描影像数据。经过后处理计算得到突触体积分数(neurite density index,NDI),神经突方向分散系数(orientation dispersion index,ODI)等NODDI指标图;以年龄、性别及IQ作为协变量,进行基于纤维束示踪的组间分析。结果 rs747302基因C/G SNP导致的被试白质组间差异主要位于双侧丘脑前辐射(anterior thalamic radiation,ATR),表现为ODI的升高及NDI的减低,且C等位基因携带者的NDI值与记忆/不分心因子呈正相关。结论 DRD4-616 SNP对于青春期正常儿童ATR白质微结构可能存在影响。

Objective: To assess the effect of-616 C/G single nucleotide polymorphism(SNP) in the dopamine D4 receptor(DRD4) promoter rs747302 on white matter microstructure in normal adolescents using neurite orientation dispersionand density imaging(NODDI) methodology. Materials and Methods: Genomic and NODDI imaging data were obtained from 179 healthy adolescents. SNP of rs747302 was genotyped. NODDI imaging data were collected uning a 3.0 tesla MR scanner, neurite density index(NDI) and orientation dispersion index(ODI) were calculated and compared between different genotype(C/G) using TBSS software. Results: Differences of NDI and ODI between genotype were found in anterior thalamic radiation(ATR), C allele carriers of rs747302 exhibited lower NDI and high ODI in ATR. Conclusions: Among male adolescents, C-allele carriers of rs747302 were associated lower NDI and high ODI in ATR.