蝉棒束孢内菌核、菌膜及其生境土壤真菌群落结构及生态功能分析

Analyses on the structure and ecological function of fungal community in sclerotia, external mycelial layer covering ossified cicada epidermis and habitat soil of Isaria cicadae

通过分析蝉棒束孢Isaria cicadae内菌核、菌膜及其生境土壤的真菌群落结构与核心真菌组的生态功能,初步探索蝉棒束孢自然生长过程中内环境真菌与其周围环境中真菌的关系。本研究利用高通量测序技术检测6株采自贵阳大将山和贵阳森林公园的蝉棒束孢及土壤样本,结果表明,蝉棒束孢内菌核共检测到43个真菌属,菌膜检测到58个真菌属,菌际土检测到260个真菌属,且内菌核和菌膜真菌群落结构相似。而两地生境土的真菌群落结构有差异,大将山以棒束孢属Isaria、被孢霉属Mortierella和一分类地位未知属占优势,而贵阳森林公园以被孢霉属、隐球酵母属Cryptococcus、红菇属Russula、棒束孢属占优势,且两地菌际土中棒束孢属的丰度均显著高于其他土样。核心真菌组检测分析显示,内菌核的核心真菌组有10个OTUs,菌膜的核心真菌组有5个OTUs,菌际土的核心真菌组有20个OTUs,3种样本的核心真菌组的生态功能群均检测到有与植物相关的功能群,内菌核中检测到外生菌根真菌群,菌膜中检测到植物病原真菌群,菌际土中检测到丛枝菌根真菌群和植物病原菌群。

The fungal community structure and ecological function of core fungal microbiome in sclerotia, external mycelial layer covering ossified cicada epidermis and habitat soil of Isaria cicadae were investigated. The relationship between fungi in internal and external environment during natural growth of Isaria cicadae was preliminarily explored. 6 Isaria cicadae isolates and soil samples collected from Mount Dajiang and Guiyang Forest Park were detected by high throughput sequencing technique. The results showed that the fungal community structure in sclerotia was similar to that in external mycelial layer covering ossified cicada epidermis. 43 genera, 58 genera and 260 genera of fungi were detected from sclerotia, external mycelial layer covering ossified cicada epidermis and soil adhering to surfaces of ossified cicada, respectively. However, the fungal community structures of habitat soil of Isaria cicadae sampled from the two sample sites were different. Isaria, Mortierella and an unclassified genus were dominant for soil sample from Mount Dajiang, while Mortierella and Cryptococcus, Russula and Isaria were dominant for soil samples from Forest Park, and the relative abundance of Isaria spp. in the soil adhering to surfaces of ossified cicada was significantly higher than that in other soil samples. The analysis of core fungal microbiome showed that 10 OTUs, 5 OTUs and 20 OTUs were detected from sclerotia, external mycelial layer covering ossified cicada epidermis and soil adhering to surfaces of ossified cicada, respectively. The ecological functional groups relative to plants were also detected. For instance, ectomycorrhizal fungi were detected from sclerotia, plant pathogens were detected from external mycelial layer covering ossified cicada epidermis, arbuscular mycorrhizal and plant saprophytic fungi were detected from soil adhering to surfaces of ossified cicada.