慢性HBV感染者血清IL-10、IL-12和IFN-γ水平的差异研究

Clinical significance and correlations of IL-10,IL-12 and IFN-γlevels in the patients with chronic hepatitis B virus infection

目的探讨不同慢性HBV感染分期及不同HBV DNA载量患者白细胞介素10(IL-10)、白细胞介素12(IL-12)和干扰素γ(IFN-γ)水平的差异。方法选择2016年3月至2017年3月于烟台市传染病医院就诊的慢性HBV感染者135例作为研究对象,其中慢性HBV携带者32例,慢性肝炎61例,肝硬化42例。选择同期40例健康体检者为对照组。采用ELISA法检测血清细胞因子IL-10、IL-12和IFN-γ水平。结果4组间IL-10和IL-12水平差异均有统计意义(F=22.06,15.67,P=0.013,0.021);Student-Newman-Keuls(SNK)法两两比较发现,肝硬化组的IL-10和IL-12水平显著高于慢性肝炎组(均P<0.001),慢性肝炎组的IL-10和IL-12水平显著高于HBV携带组(均P<0.001)。采用方差分析进行IFN-γ水平组间差异比较,结果显示,4组间IFN-γ水平差异有统计学意义(F=18.36,P=0.017)。SNK两两比较发现,其中肝硬化组的IFN-γ水平显著高于慢性肝炎组(P=0.027),慢性肝炎组的IFN-γ水平显著高于HBV携带组(P<0.001),HBV携带组的IFN-γ水平显著高于对照组(P<0.001)。HBV-DNA载量越高,IL-10水平越高,而IL-12和IFN-γ水平越低。血清IL-10、IL-12和IFN-γ水平在低、中、高HBV-DNA载量组的差异均有统计学意义(F=18.27,21.65,19.66,P=0.017,0.013,0.015)。pearson相关分析结果显示,IL-10与IFN-γ无相关性(r=0.103,P=0.063),IL-12与IFN-γ具有显著正相关性(r=0.687,P<0.05)。结论细胞因子IL-10、IL-12和IFN-γ在慢性HBV感染过程中起重要作用。

Objective To determine the changes in peripheral plasma concentrations of interleukin-10(IL-10),interleukin-12(IL-12)and interfoeron-γ(IFN-γ)in the patients with chronic hepatitis B virus(HBV)infection and their correlations with HBV infection stage or HBV DNA load of HBV carriers.Methods Data of 135 patients with chronic HBV infection from March 2016 to March 2017 were collected,the patients included 32 chronic HBV carriers,61 with chronic hepatitis and 42 with cirrhosis.Forty healthy subjects served as controls.The concentrations of IL-10,IL-12 and IFN-γwere determined using enzyme-linked immunosorbent assay(ELISA).Correlation analysis was performed using the Pearson correlation test,which was performed to analyze the correlation between IL-10,IL-12,IFN-γand HBV infection stage,HBV DNA load of HBV carriers.Results Compared with those in healthy controls,plasma IL-10 and IL-12 levels in patients with chronic hepatitis and cirrhosis increased significantly(F=22.06,15.67,P=0.013,0.021),plasma IL-10 and IL-12 levels in cirrhosis cases were higher than those in chronic hepatitis(all P<0.001),plasma IL-10 and IL-12 levels in chronic hepatitis were higher than those in chronic HBV carriers(all P<0.001).Plasma IFN-γlevel in chronic HBV carriers,chronic hepatitis and cirrhosis were significantly higher than those in healthy controls(F=18.36,P=0.017).There were statistically significant differences in IFN-γlevels among the three groups in the chronic HBV carriers,chronic hepatitis and cirrhosis.IL-10,IL-12 and IFN-γlevels of the low,medium and high HBV DNA load groups were statistically significant(all P<0.05).There was no correlation between IL-10 and HBV DNA.IFN-γ,IL-12 and HBV DNA load were negatively correlated.There was no correlation between IL-10 and IFN-γ(r=0.103,P>0.05),IL-12 and IFN-γwere significantly positively correlated(r=0.687,P<0.05).Conclusions IL-10,IL-12 and IFN-γmay play an important role in the chronic HBV infection.