异硫氰酸苄酯、乳酸链球菌素和麝香草酚复配物的抑菌活性

Antibacterial Effects of Benzyl Isothiocynate, Nisin and Thymol Compounds

为提高天然活性成分对食源性致病菌金黄色葡萄球菌和大肠杆菌的抑菌效果,本研究利用棋盘法将异硫氰酸苄酯、乳酸链球菌素和麝香草酚两两之间进行复配,并结合基于LA非参数模型的FICI模型和BI非参数模型的ΔE模型对抑菌结果进行评价。结果表明,异硫氰酸苄酯复配物-乳酸链球菌素的FICI值为0.2917<0.5,ΣΔE值的绝对值为451.74%>200%,评价结果为强协同作用;乳酸链球菌素-麝香草酚复配物的FICI值为0.5833>0.5,ΣΔE值的绝对值为19.73%<100%,评价结果为弱拮抗作用;异硫氰酸苄酯-麝香草酚复配物的FICI值为1.5000>0.5,ΣΔE值的绝对值为219.86%>200%,评价结果为强拮抗作用。总之,除了异硫氰酸苄酯-乳酸链球菌素复配物对金黄色葡萄球菌ATCC25923具有强协同抑菌作用外,其他组合无论是对金黄色葡萄球菌ATCC25923还是大肠杆菌MG1655的协同抑菌效果均不明显。

In order to enhance the antimicrobial effect of natural active ingredient on food-borne pathogen,such as Staphylococcus aureus and Escherichia coli,benzyl isothiocynate,nisin and thymol were mixed according to the chessboard method in this study,and the antibacterial effects were assessed using a FICI model based on a non-parametric model of Loewe additivity model andΔE model based on non-parametric model of Bliss independence model.Results showed that,FICI value and absolute value ofΣΔE of BITC-nisin compound were 0.2917<0.5 and 451.74%>200%,respectively,representing strong synergy.FICI value and absolute value ofΣΔE of nisin-thymol compound were 0.5833>0.5 and 19.73%<100%,respectively,representing weak antagonism.FICI value and absolute value ofΣΔE of BITC-thymol compound were 1.5000>0.5 and 219.86%>200%,respectively,representing strong antagonism.In a word,statistically significant synergy was found between nisin and BITC in their inhibitory effects on S.aureus ATCC25923,while in the other interactions synergistic antimicrobial effects on S.aureus ATCC25923 or E.coli MG1655 was not significant.