Graves病患者外周血单个核细胞中microRNA-142-3p的表达水平及其意义

Expression of microRNA-142-3p in Peripheral Blood Mononuclear Cells of Patients with Graves Disease

目的比较微小RNA-142-3p(microRNA-142-3p,miR-142-3p)在Graves病(Graves′disease,GD)患者和正常健康对照者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达水平变化,探讨miR-142-3p在Graves病发生、发展中的可能作用和机制。方法用密度梯度离心法分别提取37例Graves病患者与15例正常健康志愿者的PBMC,用RT-qPCR分别检测两组PBMC中miR-142-3p的表达水平。分析miR-142-3p水平与TT 3、TT 4和TRAb的相关性。结果miR-142-3p在GD患者PBMC中表达水平(0.378±0.199)较正常健康对照组(1.104±0.421)明显下调,差异有统计学意义(P=0.003)。miR-142-3p与TRAb、TT 4、TT 3呈负相关(r=-0.364,P=0.027;r=-0.437,P=0.007;r=-0.337,P=0.042)。同时,通过分析受试者工作特征(receiver operating characteristic,ROC)曲线发现:miR-142-3p对Graves病有一定的诊断价值,ROC曲线下面积(AUC)=0.917(95%CI:0.806~1.028)。结论miR-142-3p表达下调与Graves病相关,在疾病的发生、发展中可能起到重要作用。

Objective To compare the expression levels of microrna-142-3p(miR-142-3p)in peripheral blood mononuclear cell(PBMC)of patients with Graves disease(GD)and healthy controls.To explore the possible role and mechanism of miR-142-3p in the occurrence and development of Graves′disease.Methods PBMC was extracted from 37 patients and 15 healthy controls by density gradient centrifugation,and the levels of miR-142-3p were detected by Taq Man real-time(RT)PCR.The correlation between miR-142-3p level and TT 3,TT 4 and TRAb was analyzed.Results The expression level of miR-142-3p in PBMC of GD patients(0.378±0.199)was significantly lower than that of normal healthy control group(1.104±0.421).The difference was statistically significant(P=0.003).MiR-142-3p was negatively correlated with TRAb,TT 4 and TT 3(r=-0.364,P=0.027;r=-0.437,P=0.007;r=-0.337,P=0.042).Meanwhile receiver operating characteristic curve(ROC)suggested that the value of miR-142-3p in diagnosis for GD was high(AUC)=0.917(95%CI:0.806-1.028).Conclusion The down-regulation of miR-142-3p expression is associated with Graves disease and may play an important role in the occurrence and development of the disease.