支气管肺发育不良早产儿血中RELM-β、VEGF表达及意义

Expression and Significance of RELM beta and Vascular Endothelial Growth Factor in Blood of Premature Infants with Bronchopulmonary Dysplasia

目的探讨早产儿生后早期血清抵抗素样分子β(RELM-β)、血管内皮生长因子(VEGF)水平动态变化与支气管肺发育不良(bronchopulmonary dysplasia,BPD)的关系。方法选取2017年1月1日~2018年7月30日在徐州医科大学附属医院NICU住院的胎龄28~32周,体重<1500g,入院后应用呼吸机支持的早产儿52例,根据是否诊断BPD分为BPD组28例,非BPD组24例。采用Western blot法以及酶联免疫吸附试验(ELISA)方法检测早产儿出生后3天内、第7天、第14天血清RELM-β、VEGF水平。结果在出生后3天内、7天、14天,两组RELM-β表达水平随时间推移逐渐升高,各时间点BPD组表达均高于非BPD组,3天内两组RELM-β表达水平比较差异无统计学意义,7天、14天两组RELM-β表达水平比较差异有统计学意义(P<0.05);在生后3天内、7天、14天,两组VEGF表达水平随时间推移逐渐升高,各时间点BPD组表达均低于非BPD组,3天内、7天、14天两组VEGF表达水平比较差异有统计学意义(P<0.05)。结论RELM-β、VEGF可能参与BPD的发生而且对BPD有早期预测作用。

Objective To investigate the relationship between serum RELM beta and vascular endothelial growth factor levels and bronchopulmonary dysplasia(BPD)in preterm infants in early postnatal period.Methods Fifty-two premature infants with gestational age of 28-32 weeks and body weight less than 1500g were selected from NICU Hospital Affiliated to Xuzhou Medical University from January 2017 to July 302018.According to the diagnosis of BPD,they were divided into BPD group(28 cases)and non-BPD group(24 cases).Western blot and ELISA were used to detect the levels of RELM beta and vascular endothelial growth factor in serum of premature infants within 3 days,7 days and 14 days after birth.Results The expression level of RELM beta in the two groups increased gradually with the passage of time within 3 days,7 days and 14 days after birth.The expression level of RELM beta in BPD group was higher than that in non-BPD group at each time point.There was no significant difference in the expression level of RELM beta between the two groups within 3 days,7 days and 14 days after birth;there was significant difference in the expression level of RELM beta between the two groups within 3 days,7 days and 14 days after birth(P<0.05).The expression level of vascular endothelial growth factor in BPD group was lower than that in non-BPD group at each time point.There was significant difference in the expression level of vascular endothelial growth factor between the two groups within 3 days,7 days and 14 days(P<0.05).Conclusion RELM beta and vascular endothelial growth factor may participate in the occurrence of BPD and play an early predictive role in BPD.