SOX9基因表达对脑胶质瘤细胞增殖能力的影响

Effect of Sox9 Gene Expression on the Proliferation of Glioma Cells

【目的】探讨哺乳动物睾丸决定因子样HMG盒9(SOX9)基因表达对脑胶质瘤细胞增殖能力的影响.【方法】选取脑胶质瘤U87细胞,随机分为对照组、空白转染组和干扰组,其中空白转染组转染慢病毒,干扰组转染SOX9-慢病毒,采用MTT法检测细胞增殖,DAPI细胞核染色检测细胞凋亡,实时荧光定量PCR检测SOX9 mRNA表达,Western blot检测SOX9、SOX2、Nestin蛋白表达.【结果】干扰组细胞SOX9 mRNA表达明显低于空白转染组、对照组,空白转染组明显低于对照组(P<0.05);干扰组细胞凋亡率明显高于空白转染组、对照组,空白转染组明显高于对照组(P<0.05).干扰组培养48h、72h时OD值明显低于空白转染组、对照组,空白转染组培养48h、72h时的OD值明显低于对照组(P<0.05).干扰组SOX9、SOX2、Nestin蛋白相对表达量明显低于空白转染组、对照组,而空白转染组SOX9、SOX27蛋白相对表达量明显低于对照组(P<0.05).【结论】SOX9基因表达可抑制脑胶质瘤细胞增殖,促进细胞凋亡,下调SOX2、Nestin蛋白表达.

【Objective】To investigate the effect of testis determinant HMG box 9(Sox9)gene expression on the proliferation of glioma cells.s.【Methods】The brain glioma U87 cells were selected and randomly divided into control group,blank transfection group and interference group.The blank transfection group was transfected with lentivirus,the interference group was transfected with Sox9 lentivirus.MTT method was used to detect cell proliferation,DAPI nuclear staining was used to detect cell apoptosis,real-time fluorescent quantitative PCR was used to detect Sox9 mRNA expression,Western blot was used to detect Sox9,Sox2,nestin protein expression.【Results】The expression of Sox9 mRNA in the interference group was significantly lower than that in the blank transfection group and the control group,the blank transfection group was significantly lower than that in the control group(P<0.05),the apoptosis rate in the interference group was significantly higher than that in the blank transfection group and the control group,and the blank transfection group was significantly higher than that in the control group(P<0.05).The OD value of the interference group at 48 h and 72 h was significantly lower than that of the blank transfection group and the control group,and that of the blank transfection group at 48 h and 72 h was significantly lower than that of the control group(P<0.05).The relative expression of Sox9,Sox2 and nestin protein in the interference group was significantly lower than that in the blank transfection group and the control group,while the relative expression of Sox9 and sox27 protein in the blank transfection group was significantly lower than that in the control group(P<0.05).【Conclusion】Sox9 gene expression can inhibit the proliferation of glioma cells,promote apoptosis and down regulate the expression of Sox2 and nestin protein.