共轭亚油酸-紫杉醇自组装纳米粒的结构分析

Structure of the Conjugated Linoleic Acid-Paclitaxel Conjugate Self-Assembled Nanoparticles

目的制备共轭亚油酸-紫杉醇自组装纳米粒(conjugated linoleic acid-paclitaxel conjugate self-assembled nanoparticles,CLA-PTX NPs)。方法本实验采用纳米沉淀法制备,通过动态光散射、核磁共振氢谱、拉曼光谱、傅里叶变换红外光谱及氮元素分布分析等方法对所制备的CLA-PTX NPs的表面和内部基团进行表征,确定CLA-PTX NPs的表面基团和内部基团,为阐述其自组装机制提供依据。结果PTX中C-1位和C-7位的羟基、C-4位和C-10位的乙酰基位于CLA-PTX NPs的表面,CLA碳链、PTX中C-2位和C-3'位的苯环及C-3'位的酰胺键位于CLA-PTX NPs的内部。据此推测CLA-PTX的自组装方式是非极性的CLA碳链因疏水作用而自发向内聚集,PTX中亲水的含氧基团羟基、羰基等在纳米粒表面从而形成纳米粒。结论CLAPTX NPs表面基团的确定也为其可能的表面修饰提供参考和依据。更为重要的是,本实验还为其他自组装纳米粒结构的表征提供了相关可行的方法。

OBJECTIVE To prepare,the conjugated linoleic acid-paclitaxel conjugate self-assembled nanoparticles(CLA-PTX NPs)by nanoprecipitation.METHODS The Dynamic light scattering,nuclear magnetic resonance spectroscopy,raman spectroscopy,fourier transform infrared spectroscopy and nitrogen element distribution of CLA-PTX NPs were studied.RESULTS The hydroxyl groups(C-4 and C-10 of PTX)and the acetyl groups(C-1 and C-7 of PTX)were on the surface of CLA-PTX NPs,CLA carbon chain,the benzene ring(C-2 and C-3'of PTX)and the amide bond(C-3'of PTX)were inside the CLA-PTX NPs.CONCLUSION It is speculated that the self-assembly of CLA-PTX is that the non-polar CLA carbon chain spontaneously aggregates inward due to hydrophobic interaction,and the hydrophilic oxygen-containing groups of PTX(hydroxyl group and carbonyl group)are on the surface of the nanoparticle to form nanoparticles.