摘要
本研究主要基于投影寻踪分类法(PPC)对引进美国甘蔗种质主要工艺品质性状进行世代演进和种质评价分析。根据育成或改良年代(10~20 a为时间界点)把引进的58份美国甘蔗种质划分4个改良世代,基于大田试验,对甘蔗锤度、甘蔗蔗糖分、蔗汁蔗糖分、甘蔗纤维分、甘蔗简纯度等5个重要工艺品质性状指标进行方差、投影寻踪分类和聚类分析,以揭示其世代演进特征和筛选工艺品质优异种质资源用于杂交育种。方差分析表明,世代内和世代间各种质资源工艺品质性状存在显著差异(P<0.05),变异系数在2%~20%之间,其中甘蔗蔗糖分、蔗汁蔗糖分和简纯度3个品质性状还存在世代和月份间的显著交互作用。投影寻踪分类法评价分析表明,不同世代美国甘蔗种质资源投影值差异显著,其中以第1代最低,依次增加,第4代最高;投影方向以甘蔗蔗糖分最高,依次为蔗汁蔗糖分和甘蔗锤度,简纯度和纤维分最低。根据投影值大小的聚类分析表明,58份种质可以划分为4类。筛选了主要工艺品质性状优良的甘蔗种质18份(第Ⅱ类)用于杂交育种,其中来自第4代的材料有6份,占第4代材料总数的46.15%;来自第3代的材料有10份,占总数的37.07%;来自第2代的材料有1份,占总数18.18%;第一代材料未筛选出品质优良种质资源。以上结果可以看出,美国甘蔗种质资源主要工艺品质改良显著,随着世代演进而显著提升。表现优异外引种质以第4代所占比重最高,依次递减,第一代最少,优异种质多来源于演进次数最多的世代;投影寻踪分类法可作为甘蔗改良效果和种质资源评价研究的另一途径。
The generational evolution and germplasm evaluation of major technological quality traits in the sugarcane germplasms introduced from the United States were analyzed based on the Projection Pursuit Classification(PPC).Fifty-eight American sugarcane germplasms were divided into four improved generations according to the breeding or improvement years(10–20 years as the time boundary).Based on field experiments,variance,projection pursuit classification and cluster analysis were carried out on five important technological quality traits such as brix,sucrose content,sugarcane juice sugar content,sugarcane fiber content and sugarcane purity to reveal the generational evolution characteristics and screen excellent germplasms for cross breeding.Variance analysis showed that there were significant differences in technological quality traits between generations and in generations(P<0.05),and the coefficient of variation was between 2%and 20%.There were significant interactions among generations and months among sugarcane sugar,sucrose content in juice and purity. PPC analysis showed that the projection values of sugarcane germplasms in differentgenerations of the United States were significantly different, among which the first generation was the lowest, increasedin turn, and the fourth generation was the highest;the projection direction was the highest sucrose content in sugarcane,followed by sucrose content in sugarcane juice and brix content in sugarcane, with the lowest purity and fiber content.Cluster analysis based on projection values showed that the germplasms could be divided into four groups. Eighteensugarcane germplasms (group II) with good technological quality traits were screened for cross breeding. Six of themcame from the fourth generation, accounting for 46.15% of the total number of materials in the fourth generation;tenfrom the third generation, accounting for 37.07% of the total number;one from the second generation, accounting for18.18% of the total number;and no good quality germplasm resources were screened from the first generation. Theabove results showed that the main technological quality of sugarcane germplasm resources in the United States hadbeen significantly improved, and had been significantly improved with the evolution of generations. Projection pursuittaxonomy could be used as another way to evaluate the improvement effect and germplasm resources of sugarcane.
作者
赵勇
赵丽萍
昝逢刚
朱建荣
覃伟
赵俊
赵培方
刘家勇
ZHAO Yong;ZHAO Liping;ZAN Fenggang;ZHU Jianrong;QIN Wei;ZHAO Jun;ZHAO Peifang;LIU Jiayong(Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory of Sugarcane Genetic Improvement,Kaiyuan,Yunnan 661699,China)
出处
《热带作物学报》
CSCD
北大核心
2019年第11期2127-2134,共8页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31660418)
“云岭英才计划”高端外国专家资助项目
云南省科技计划青年项目(No.2017FD022)
关键词
美国
甘蔗种质
世代演进
投影寻踪分类法
聚类分析
United States
sugarcane germplasm
generation succession
projection pursuit cluster
cluster analysis